Autor segons l'article: Quinones, Mar; Sanchez, David; Muguerza, Begona; Miguel, Marta; Aleixandre, Amaya
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Muguerza Marquínez, Maria Begoña
Paraules clau: Angiotensin converting enzyme; Cocoa; Nitric oxide; Oxidative stress; Polyphenols
Resum: We investigate the mechanisms involved in the long-term antihypertensive effect of a polyphenol-rich cocoa powder, named CocoanOX® (CCX), in spontaneously hypertensive rats (SHR). We have carried out two different batches of experiments. For the first batch of experiments, forty 3 week-old male SHR were randomly divided with ad libitum intake into four groups of 10 animals, that respectively received the following drinking fluids up to the 20th week of life (treatment period): tap water (control), CCX 100 mg/kg/day, CCX 200 mg/kg/day and CCX 400 mg/kg/day. Five 20 week-old rats of each group were sacrificed by decapitation. From the 20th to 24th week of life all the remaining animals were given tap water (follow-up period), and all of them were sacrificed at the end of the follow-up period. Plasma malondialdehyde (MDA), reduced glutathione in the liver, plasma and aorta angiotensin converting enzyme (ACE) activity and plasma angiotensin II were determined in all the sacrificed SHR that were included in this batch of experiments. Plasma MDA decreased and liver reduced glutathione increased in the 20 week-old CCX treated SHR. These effects were not observed in the rats that were sacrificed after the follow-up period. CCX treatment did not modify aorta ACE activity, but the activity of ACE and the levels of angiotensin II increased in the plasma of the SHR treated with the highest dose of CCX. ACE activity returned to basal values in the SHR that were sacrificed after the follow-up period. However, angiotensin II levels were slightly higher after withdrawal of CCX.For the second batch of experiments we used aorta rings obtained from untreated SHR, and we evaluated the relaxation caused by CCX in different aorta preparations. CCX relaxed the intact aorta preparations but this cocoa did not relax the endothelium-denuded aorta rings from the untreated SHR. l-NAME, but not indomethacin, inhibited the relaxation caused by CCX in the SHR aorta rings. We postulate that the antihypertensive effect of CCX might be mediated by an improvement of endothelial release of nitric oxide and by a reduction of oxidative stress. The inhibition of ACE could be implicated in the antihypertensive effect of CCX
Àrees temàtiques: Administração pública e de empresas, ciências contábeis e turismo; Astronomia / física; Biodiversidade; Biotecnología; Ciência da computação; Ciência de alimentos; Ciências agrárias i; Ciências ambientais; Ciências biológicas i; Ciências biológicas ii; Ciências biológicas iii; Educação; Engenharias i; Engenharias ii; Engenharias iii; Engenharias iv; Ensino; Farmacia; Food science; Food science & technology; Interdisciplinar; Matemática / probabilidade e estatística; Materiais; Medicina i; Medicina ii; Medicina veterinaria; Nutrição; Odontología; Química; Saúde coletiva; Zootecnia / recursos pesqueiros
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: begona.muguerza@urv.cat
Data d'alta del registre: 2025-01-25
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/pii/S0963996910004072?via%3Dihub
Referència a l'article segons font original: Food Research International. 44 (5): 1203-1208
Referència de l'ítem segons les normes APA: Quinones, Mar; Sanchez, David; Muguerza, Begona; Miguel, Marta; Aleixandre, Amaya (2011). Mechanisms for antihypertensive effect of CocoanOX, a polyphenol-rich cocoa powder, in spontaneously hypertensive rats. Food Research International, 44(5), 1203-1208. DOI: 10.1016/j.foodres.2010.10.032
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1016/j.foodres.2010.10.032
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2011
Tipus de publicació: Journal Publications
Repositori URV