Autor segons l'article: Lopez-Malo, Maria; Chiva, Rosana; Rozes, Nicolas; Guillamon, José Manuel;
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Rozès, Nicolas Andre Louis
Paraules clau: Yeast Overexpressing strains Mutant Lipids Cold
Resum: The growing demand for wines with a more pronounced aromatic profile calls for low temperature alcoholic fermentations (10-15 °C). However, there are certain drawbacks to low temperature fermentations such as reduced growth rate, long lag phase and sluggish or stuck fermentations. The lipid metabolism of Saccharomyces cerevisiae plays a central role in low temperature adaptation. The aim of this study was to detect lipid metabolism genes involved in cold adaptation. To do so, we analyzed the growth of knockouts in phospholipids, sterols and sphingolipids, from the EUROSCARF collection S. cerevisiae BY4742 strain at low and optimal temperatures. Growth rate of these knockouts, compared with the control, enabled us to identify the genes involved, which were also deleted or overexpressed in a derivative haploid of a commercial wine strain. We identified genes involved in the phospholipid (PSD1 and OPI3), sterol (ERG3 and IDI1) and sphingolipid (LCB3) pathways, whose deletion strongly impaired growth at low temperature and whose overexpression reduced generation or division time by almost half. Our study also reveals many phenotypic differences between the laboratory strain and the commercial wine yeast strain, showing the importance of constructing mutant and overexpressing strains in both genetic backgrounds. The phenotypic differences in the mutant and overexpressing strains were correlated with changes in their lipid composition.
Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Safety, risk, reliability and quality Química Odontología Nutrição Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geociências General medicine Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: nicolasrozes@urv.cat
Identificador de l'autor: 0000-0001-9718-3429
Data d'alta del registre: 2024-04-20
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0168160512006617
Referència a l'article segons font original: International Journal Of Food Microbiology. 162 (1): 26-36
Referència de l'ítem segons les normes APA: Lopez-Malo, Maria; Chiva, Rosana; Rozes, Nicolas; Guillamon, José Manuel; (2013). Phenotypic analysis of mutant and overexpressing strains of lipid metabolism genes in Saccharomyces cerevisiae: Implication in growth at low temperatures. International Journal Of Food Microbiology, 162(1), 26-36. DOI: 10.1016/j.ijfoodmicro.2012.12.020
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1016/j.ijfoodmicro.2012.12.020
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2013
Tipus de publicació: Journal Publications
Repositori URV