Autor segons l'article: Pazos, Elena; Garcia-Algar, Manuel; Penas, Cristina; Nazarenus, Moritz; Torruella, Arnau; Pazos-Perez, Nicolas; Guerrini, Luca; Eugenio Vazquez, M; Garcia-Rico, Eduardo; Mascarenas, Jose L; Alvarez-Puebla, Ramon A
Departament: Química Física i Inorgànica
Autor/s de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Pazos Pérez, Nicolás Carlos
Paraules clau: Surface enhanced raman scattering Spectroscopy Silver surfaces Sers detection Self-assembled monolayers Raman scattering Protein Path Gold Cells Cancer Biopsy
Resum: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.
Àrees temàtiques: Química Materiais Interdisciplinar General chemistry Farmacia Engenharias iv Engenharias iii Engenharias ii Colloid and surface chemistry Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemistry Catalysis Biochemistry Astronomia / física
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: ramon.alvarez@urv.cat luca.guerrini@urv.cat nicolas.pazos@urv.cat
Identificador de l'autor: 0000-0003-4770-5756 0000-0002-2925-1562 0000-0002-2326-4231
Data d'alta del registre: 2024-11-02
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://pubs.acs.org/doi/10.1021/jacs.6b08957
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Journal Of The American Chemical Society. 138 (43): 14206-14209
Referència de l'ítem segons les normes APA: Pazos, Elena; Garcia-Algar, Manuel; Penas, Cristina; Nazarenus, Moritz; Torruella, Arnau; Pazos-Perez, Nicolas; Guerrini, Luca; Eugenio Vazquez, M; Ga (2016). Surface-Enhanced Raman Scattering Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC. Journal Of The American Chemical Society, 138(43), 14206-14209. DOI: 10.1021/jacs.6b08957
DOI de l'article: 10.1021/jacs.6b08957
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2016
Tipus de publicació: Journal Publications
Repositori URV