Articles producció científica> Química Física i Inorgànica

Surface-Enhanced Raman Scattering Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC

  • Dades identificatives

    Identificador: imarina:5130463
    Handle: https://hdl.handle.net/20.500.11797/imarina5130463
    Autors:
    Pazos, ElenaGarcia-Algar, ManuelPenas, CristinaNazarenus, MoritzTorruella, ArnauPazos-Perez, NicolasGuerrini, LucaEugenio Vazquez, MGarcia-Rico, EduardoMascarenas, Jose LAlvarez-Puebla, Ramon A
    Resum:
    Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.

  • Altres:

    Autor segons l'article: Pazos, Elena; Garcia-Algar, Manuel; Penas, Cristina; Nazarenus, Moritz; Torruella, Arnau; Pazos-Perez, Nicolas; Guerrini, Luca; Eugenio Vazquez, M; Garcia-Rico, Eduardo; Mascarenas, Jose L; Alvarez-Puebla, Ramon A
    Departament: Química Física i Inorgànica
    Autor/s de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Pazos Pérez, Nicolás Carlos
    Paraules clau: Surface enhanced raman scattering Spectroscopy Silver surfaces Sers detection Self-assembled monolayers Raman scattering Protein Path Gold Cells Cancer Biopsy
    Resum: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.
    Àrees temàtiques: Química Materiais Interdisciplinar General chemistry Farmacia Engenharias iv Engenharias iii Engenharias ii Colloid and surface chemistry Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemistry Catalysis Biochemistry Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: ramon.alvarez@urv.cat luca.guerrini@urv.cat nicolas.pazos@urv.cat
    Identificador de l'autor: 0000-0003-4770-5756 0000-0002-2925-1562 0000-0002-2326-4231
    Data d'alta del registre: 2024-11-02
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Journal Of The American Chemical Society. 138 (43): 14206-14209
    Referència de l'ítem segons les normes APA: Pazos, Elena; Garcia-Algar, Manuel; Penas, Cristina; Nazarenus, Moritz; Torruella, Arnau; Pazos-Perez, Nicolas; Guerrini, Luca; Eugenio Vazquez, M; Ga (2016). Surface-Enhanced Raman Scattering Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC. Journal Of The American Chemical Society, 138(43), 14206-14209. DOI: 10.1021/jacs.6b08957
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2016
    Tipus de publicació: Journal Publications

  • Paraules clau:

    Biochemistry,Catalysis,Chemistry,Chemistry (Miscellaneous),Chemistry, Multidisciplinary,Colloid and Surface Chemistry
    Surface enhanced raman scattering
    Spectroscopy
    Silver surfaces
    Sers detection
    Self-assembled monolayers
    Raman scattering
    Protein
    Path
    Gold
    Cells
    Cancer
    Biopsy
    Química
    Materiais
    Interdisciplinar
    General chemistry
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Colloid and surface chemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Chemistry
    Catalysis
    Biochemistry
    Astronomia / física

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