Author, as appears in the article.: Pazos, Elena; Garcia-Algar, Manuel; Penas, Cristina; Nazarenus, Moritz; Torruella, Arnau; Pazos-Perez, Nicolas; Guerrini, Luca; Eugenio Vazquez, M; Garcia-Rico, Eduardo; Mascarenas, Jose L; Alvarez-Puebla, Ramon A
Department: Química Física i Inorgànica
URV's Author/s: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Pazos Pérez, Nicolás Carlos
Keywords: Surface enhanced raman scattering Spectroscopy Silver surfaces Sers detection Self-assembled monolayers Raman scattering Protein Path Gold Cells Cancer Biopsy
Abstract: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.
Thematic Areas: Química Materiais Interdisciplinar General chemistry Farmacia Engenharias iv Engenharias iii Engenharias ii Colloid and surface chemistry Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemistry Catalysis Biochemistry Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: ramon.alvarez@urv.cat luca.guerrini@urv.cat nicolas.pazos@urv.cat
Author identifier: 0000-0003-4770-5756 0000-0002-2925-1562 0000-0002-2326-4231
Record's date: 2024-11-02
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://pubs.acs.org/doi/10.1021/jacs.6b08957
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of The American Chemical Society. 138 (43): 14206-14209
APA: Pazos, Elena; Garcia-Algar, Manuel; Penas, Cristina; Nazarenus, Moritz; Torruella, Arnau; Pazos-Perez, Nicolas; Guerrini, Luca; Eugenio Vazquez, M; Ga (2016). Surface-Enhanced Raman Scattering Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC. Journal Of The American Chemical Society, 138(43), 14206-14209. DOI: 10.1021/jacs.6b08957
Article's DOI: 10.1021/jacs.6b08957
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV