Autor segons l'article: Ramadurai S, Werner M, Slater NKH, Martin A, Baulin VA, Keyes TE.
Departament: Enginyeria Química
e-ISSN: 1744-6848
Autor/s de la URV: Baulin, Vladimir
Paraules clau: Poly(l-lysine iso-phthalamide) Peptides Model membranes Lifetime correlation spectroscopy Lateral diffusion Hydrophobic mismatch Fluorescence correlation spectroscopy Cell-membrane Blood-cells Bilayer-membranes
Resum: Deeper understanding of the molecular interactions between polymeric materials and the lipid membrane is important across a range of applications from permeation for drug delivery to encapsulation for immuno-evasion. Using highly fluidic microcavity supported lipid bilayers, we studied the interactions between amphiphilic polymer PP50 and a DOPC lipid bilayer. As the PP50 polymer is pH responsive the studies were carried out at pH 6.5, 7.05 and 7.5, corresponding to fully, partly protonated (pH = pKa = 7.05) and fully ionized states of the polymer, respectively. Fluorescence correlation spectroscopy (FCS) using both labelled lipid and polymer revealed the PP50 associates with the bilayer interface across all pHs where its diffusion along the interface is impeded. Both FCS and electrochemical impedance spectroscopy (EIS) data indicate that the PP50 does not penetrate fully into the bilayer core but rather forms a layer at the bilayer aqueous interface reflected in increased resistance and decreased capacitance of the bilayer on PP50 binding. The extent of these effects and the dynamics of binding are influenced by pH, increasing with decreasing pH. These experimental trends concurred with coarse grained Monte Carlo simulations of polymer-bilayer interactions wherein a model hydrophilic polymer backbone grafted with side chains of varying hydrophobicity, to mimic the effect of varying pH, was simulated based on the bond fluctuation model with explicit solvent. Simulation results showed that with increasing hydrophobicity, the polymer penetrated deeper into the contacting bilayer leaflet of the membrane suppressing, consistent with EIS data, solvent permeation and that a full insertion of the polymer into the bilayer core is not necessary for suppression of permeability.
Àrees temàtiques: Química Polymer science Physics, multidisciplinary Materials science, multidisciplinary Materiais Interdisciplinar General chemistry Farmacia Engenharias iv Engenharias iii Engenharias ii Condensed matter physics Ciências biológicas ii Ciências biológicas i Ciências agrárias i Chemistry, physical Chemistry (miscellaneous) Chemistry (all) Biotecnología Astronomia / física
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1744-683X
Adreça de correu electrònic de l'autor: vladimir.baulin@urv.cat
Identificador de l'autor: 0000-0003-2086-4271
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://pubs.rsc.org/en/content/articlelanding/2017/sm/c6sm02645a#!divAbstract
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Soft Matter. 13 (20): 3690-3700
Referència de l'ítem segons les normes APA: Ramadurai S, Werner M, Slater NKH, Martin A, Baulin VA, Keyes TE. (2017). Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane. Soft Matter, 13(20), 3690-3700. DOI: 10.1039/C6SM02645A
DOI de l'article: 10.1039/c6sm02645a
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Tipus de publicació: Journal Publications