Autor segons l'article: Sunyer-Figueres M., Wang C., Mas A.
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Mas Baron, Alberto
Paraules clau: Wine yeasts Heat Dimethyl dicarbonate (dmdc) Cell lysis heat dimethyl dicarbonate (dmdc) cell lysis
Resum: During wine production, some yeasts enter a Viable But Not Culturable (VBNC) state, which may influence the quality and stability of the final wine through remnant metabolic activity or by resuscitation. Culture-independent techniques are used for obtaining an accurate estimation of the number of live cells, and quantitative PCR could be the most accurate technique. As a marker of cell viability, rRNA was evaluated by analyzing its stability in dead cells. The species-specific stability of rRNA was tested in Saccharomyces cerevisiae, as well as in three species of non-Saccharomyces yeast (Hanseniaspora uvarum, Torulaspora delbrueckii and Starmerella bacillaris). High temperature and antimicrobial dimethyl dicarbonate (DMDC) treatments were efficient in lysing the yeast cells. rRNA gene and rRNA (as cDNA) were analyzed over 48 h after cell lysis by quantitative PCR. The results confirmed the stability of rRNA for 48 h after the cell lysis treatments. To sum up, rRNA may not be a good marker of cell viability in the wine yeasts that were tested.
Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Safety, risk, reliability and quality Química Odontología Nutrição Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geociências General medicine Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
ISSN: 01681605
Adreça de correu electrònic de l'autor: albert.mas@urv.cat
Identificador de l'autor: 0000-0002-0763-1679
Pàgina final: 4
Data d'alta del registre: 2024-09-07
Volum de revista: 270
Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0168160518300369
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: International Journal Of Food Microbiology. 270 1-4
Referència de l'ítem segons les normes APA: Sunyer-Figueres M., Wang C., Mas A. (2018). Analysis of ribosomal RNA stability in dead cells of wine yeast by quantitative PCR. International Journal Of Food Microbiology, 270(), 1-4. DOI: 10.1016/j.ijfoodmicro.2018.01.020
DOI de l'article: 10.1016/j.ijfoodmicro.2018.01.020
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2018
Pàgina inicial: 1
Tipus de publicació: Journal Publications
Repositori URV