Autor segons l'article: Magrina, Ivan; Jauset-Rubio, Miriam; Ortiz, Mayreli; Tomaso, Herbert; Simonova, Anna; Hocek, Michal; O'Sullivan, Ciara K
Departament: Enginyeria Química
Autor/s de la URV: Jauset Rubio, Miriam / Magriñá Lobato, Ivan / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli
Paraules clau: Triphosphates Redox label Real Number Nucleotides Identification Gene Francisella-tularensis Assays Amplification
Resum: We report the duplex amplification of two plasmid DNA markers involved in the virulence of Bacillus anthracis, CAP and PAG, and the direct electrochemical detection of these amplicons. The method consists of the simultaneous amplification of the two targets in a single-pot reaction via polymerase chain reaction (PCR) using tailed primers and ferrocene-labeled dATP. Following amplification, the PCR products hybridize to probes immobilized on electrodes in a microfabricated electrode array chip. The incorporated ferrocene labeled dATP is then detected using square wave voltammetry. We evaluated the effect of electrolyte cations, anions, and concentration to condense, bend, and shrink double-stranded DNA and their effect on the intensity of the ferrocene signal. We obtained detection limits 40.8 and 3.4 fM for CAP and PAG targets, respectively. We successfully developed a method to detect the presence of both targets in genomic DNA extracted from real samples.
Àrees temàtiques: Química Interdisciplinar General chemistry General chemical engineering Engenharias ii Ciências agrárias i Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemical engineering (miscellaneous) Chemical engineering (all)
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 24701343
Adreça de correu electrònic de l'autor: mayreli.ortiz@urv.cat miriam.jauset@urv.cat mayreli.ortiz@urv.cat
Identificador de l'autor: 0000-0002-9423-0055 0000-0002-9943-6132 0000-0002-9423-0055
Data d'alta del registre: 2024-10-26
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://pubs.acs.org/doi/abs/10.1021/acsomega.9b02890
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Acs Omega. 4 (26): 21900-21908
Referència de l'ítem segons les normes APA: Magrina, Ivan; Jauset-Rubio, Miriam; Ortiz, Mayreli; Tomaso, Herbert; Simonova, Anna; Hocek, Michal; O'Sullivan, Ciara K (2019). Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP. Acs Omega, 4(26), 21900-21908. DOI: 10.1021/acsomega.9b02890
DOI de l'article: 10.1021/acsomega.9b02890
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2019
Tipus de publicació: Journal Publications
Repositori URV