Autor segons l'article: Samarra, Iris; Ramos-Molina, Bruno; Isabel Queipo-Ortuno, M.; Tinahones, Francisco J.; Arola, Lluis; Delpino-Rius, Antoni; Herrero, Pol; Canela, Nuria;
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Arola Ferrer, Luis Maria / HERRERO GIL, POL
Paraules clau: Quantification Polyamines Polyamine metabolism Obesity N-acetylated polyamines Ms Liquid biopsies Lc-ms/ms Lc-ms Human urine Electrospray-ionization Derivatives Dansylated polyamines Chromatography-mass spectrometry Chloride derivatization Cancer-patients Benzoyl chloride Acetylpolyamines polyamine metabolism obesity liquid biopsies lc-ms/ms acetylpolyamines
Resum: Polyamines are involved in the regulation of many cellular functions and are promising biomarkers of numerous physiological conditions. Since the concentrations of these compounds in biological fluids are low, sample extraction is one of the most critical steps of their analysis. Here, we developed a comprehensive, sensitive, robust, and high-throughput LC-MS/MS stable-isotope dilution method for the simultaneous determination of 19 metabolites related to polyamine metabolism, including polyamines, acetylated and diacetylated polyamines, precursors, and catabolites from liquid biopsies. The sample extraction was optimized to remove interfering compounds and to reduce matrix effects, thus being useful for large clinical studies. The method consists of two-step liquid-liquid extraction with a Folch extraction and ethyl acetate partitioning combined with dansyl chloride derivatization. The developed method was applied to a small gender-related trial concerning human serum and urine samples from 40 obese subjects. Sex differences were found for cadaverine, putrescine, 1,3-diaminopropane, gamma-aminobutyric acid, N8-acetylspermidine, and N-acetylcadaverine in urine; N1-acetylspermine in serum; and spermine in both serum and urine. The results demonstrate that the developed method can be used to analyze biological samples for the study of polyamine metabolism and its association with human diseases.
Àrees temàtiques: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2218273X
Adreça de correu electrònic de l'autor: lluis.arola@urv.cat
Identificador de l'autor: 0000-0003-2767-1974
Data d'alta del registre: 2023-02-18
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.mdpi.com/2218-273X/9/12/779
Referència a l'article segons font original: Biomolecules. 9 (12):
Referència de l'ítem segons les normes APA: Samarra, Iris; Ramos-Molina, Bruno; Isabel Queipo-Ortuno, M.; Tinahones, Francisco J.; Arola, Lluis; Delpino-Rius, Antoni; Herrero, Pol; Canela, Nuria (2019). Gender-Related Differences on Polyamine Metabolome in Liquid Biopsies by a Simple and Sensitive Two-Step Liquid-Liquid Extraction and LC-MS/MS. Biomolecules, 9(12), -. DOI: 10.3390/biom9120779
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.3390/biom9120779
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2019
Tipus de publicació: Journal Publications