Articles producció científica> Bioquímica i Biotecnologia

Gender-Related Differences on Polyamine Metabolome in Liquid Biopsies by a Simple and Sensitive Two-Step Liquid-Liquid Extraction and LC-MS/MS

  • Datos identificativos

    Identificador: imarina:6040290
    Autores:
    Samarra, IrisRamos-Molina, BrunoIsabel Queipo-Ortuno, M.Tinahones, Francisco J.Arola, LluisDelpino-Rius, AntoniHerrero, PolCanela, Nuria
    Resumen:
    Polyamines are involved in the regulation of many cellular functions and are promising biomarkers of numerous physiological conditions. Since the concentrations of these compounds in biological fluids are low, sample extraction is one of the most critical steps of their analysis. Here, we developed a comprehensive, sensitive, robust, and high-throughput LC-MS/MS stable-isotope dilution method for the simultaneous determination of 19 metabolites related to polyamine metabolism, including polyamines, acetylated and diacetylated polyamines, precursors, and catabolites from liquid biopsies. The sample extraction was optimized to remove interfering compounds and to reduce matrix effects, thus being useful for large clinical studies. The method consists of two-step liquid-liquid extraction with a Folch extraction and ethyl acetate partitioning combined with dansyl chloride derivatization. The developed method was applied to a small gender-related trial concerning human serum and urine samples from 40 obese subjects. Sex differences were found for cadaverine, putrescine, 1,3-diaminopropane, gamma-aminobutyric acid, N8-acetylspermidine, and N-acetylcadaverine in urine; N1-acetylspermine in serum; and spermine in both serum and urine. The results demonstrate that the developed method can be used to analyze biological samples for the study of polyamine metabolism and its association with human diseases.
  • Otros:

    Autor según el artículo: Samarra, Iris; Ramos-Molina, Bruno; Isabel Queipo-Ortuno, M.; Tinahones, Francisco J.; Arola, Lluis; Delpino-Rius, Antoni; Herrero, Pol; Canela, Nuria;
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: Arola Ferrer, Luis Maria / HERRERO GIL, POL
    Palabras clave: Quantification Polyamines Polyamine metabolism Obesity N-acetylated polyamines Ms Liquid biopsies Lc-ms/ms Lc-ms Human urine Electrospray-ionization Derivatives Dansylated polyamines Chromatography-mass spectrometry Chloride derivatization Cancer-patients Benzoyl chloride Acetylpolyamines polyamine metabolism obesity liquid biopsies lc-ms/ms acetylpolyamines
    Resumen: Polyamines are involved in the regulation of many cellular functions and are promising biomarkers of numerous physiological conditions. Since the concentrations of these compounds in biological fluids are low, sample extraction is one of the most critical steps of their analysis. Here, we developed a comprehensive, sensitive, robust, and high-throughput LC-MS/MS stable-isotope dilution method for the simultaneous determination of 19 metabolites related to polyamine metabolism, including polyamines, acetylated and diacetylated polyamines, precursors, and catabolites from liquid biopsies. The sample extraction was optimized to remove interfering compounds and to reduce matrix effects, thus being useful for large clinical studies. The method consists of two-step liquid-liquid extraction with a Folch extraction and ethyl acetate partitioning combined with dansyl chloride derivatization. The developed method was applied to a small gender-related trial concerning human serum and urine samples from 40 obese subjects. Sex differences were found for cadaverine, putrescine, 1,3-diaminopropane, gamma-aminobutyric acid, N8-acetylspermidine, and N-acetylcadaverine in urine; N1-acetylspermine in serum; and spermine in both serum and urine. The results demonstrate that the developed method can be used to analyze biological samples for the study of polyamine metabolism and its association with human diseases.
    Áreas temáticas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 2218273X
    Direcció de correo del autor: lluis.arola@urv.cat
    Identificador del autor: 0000-0003-2767-1974
    Fecha de alta del registro: 2023-02-18
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2218-273X/9/12/779
    Referencia al articulo segun fuente origial: Biomolecules. 9 (12):
    Referencia de l'ítem segons les normes APA: Samarra, Iris; Ramos-Molina, Bruno; Isabel Queipo-Ortuno, M.; Tinahones, Francisco J.; Arola, Lluis; Delpino-Rius, Antoni; Herrero, Pol; Canela, Nuria (2019). Gender-Related Differences on Polyamine Metabolome in Liquid Biopsies by a Simple and Sensitive Two-Step Liquid-Liquid Extraction and LC-MS/MS. Biomolecules, 9(12), -. DOI: 10.3390/biom9120779
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.3390/biom9120779
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2019
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry,Biochemistry & Molecular Biology,Molecular Biology
    Quantification
    Polyamines
    Polyamine metabolism
    Obesity
    N-acetylated polyamines
    Ms
    Liquid biopsies
    Lc-ms/ms
    Lc-ms
    Human urine
    Electrospray-ionization
    Derivatives
    Dansylated polyamines
    Chromatography-mass spectrometry
    Chloride derivatization
    Cancer-patients
    Benzoyl chloride
    Acetylpolyamines
    polyamine metabolism
    obesity
    liquid biopsies
    lc-ms/ms
    acetylpolyamines
    Química
    Molecular biology
    Materiais
    General medicine
    Farmacia
    Ensino
    Biochemistry & molecular biology
    Biochemistry
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