Autor segons l'article: Rajeev G; Melville E; Cowin AJ; Prieto-Simon B; Voelcker NH
Departament: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/s de la URV: Prieto Simón, Beatriz
Paraules clau: Repair Porous alumina Nanohole arrays Immunosensing Flii Flightless-i Flightless detection Electrochemical biosensor Chronic wound Attenuation Actin-remodeling protein
Resum: © Copyright © 2020 Rajeev, Melville, Cowin, Prieto-Simon and Voelcker. A label-free electrochemical detection platform for the sensitive and rapid detection of Flightless I (Flii) protein, a biomarker of wound chronicity, has been developed using nanoporous anodic alumina (NAA) membranes modified with Flii antibody recognition sites. The electrochemical detection is based on the nanochannel blockage experienced upon Flii capture by immobilized antibodies within the nanochannels. This capture impedes the diffusion of redox species [[Fe(CN)6]4−/3−] toward a gold electrode attached at the backside of the modified NAA membrane. Partial blockage causes a decrease in the oxidation current of the redox species at the electrode surface which is used as an analytical signal by the reported biosensor. The resulting biosensing system allows detection of Flii at the levels found in wounds. Two types of assays were tested, sandwich and direct, showing <3 and 2 h analysis time, respectively, a significant reduction in time from the nearly 48 h required for the conventional Western blot assay. Slightly higher sensitivity values were observed for the sandwich-based strategy. With faster analysis, lack of matrix effects, robustness, ease of use and cost-effectiveness, the developed sensing platform has the potential to be translated into a point-of-care (POC) device for chronic wound management and as a simple alternative characterization tool in Flii research.
Àrees temàtiques: Química Medicina ii Medicina i Materiais Interdisciplinar General chemistry Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: beatriz.prieto-simon@urv.cat
Identificador de l'autor: 0000-0001-8016-1565
Data d'alta del registre: 2023-02-19
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.frontiersin.org/articles/10.3389/fchem.2020.00155/full
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Frontiers In Chemistry. 8 (155):
Referència de l'ítem segons les normes APA: Rajeev G; Melville E; Cowin AJ; Prieto-Simon B; Voelcker NH (2020). Porous Alumina Membrane-Based Electrochemical Biosensor for Protein Biomarker Detection in Chronic Wounds. Frontiers In Chemistry, 8(155), -. DOI: 10.3389/fchem.2020.00155
DOI de l'article: 10.3389/fchem.2020.00155
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2020
Tipus de publicació: Journal Publications
Repositori URV