Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Porous Alumina Membrane-Based Electrochemical Biosensor for Protein Biomarker Detection in Chronic Wounds

  • Dades identificatives

    Identificador: imarina:6232132
    Autors:
    Rajeev GMelville ECowin AJPrieto-Simon BVoelcker NH
    Resum:
    © Copyright © 2020 Rajeev, Melville, Cowin, Prieto-Simon and Voelcker. A label-free electrochemical detection platform for the sensitive and rapid detection of Flightless I (Flii) protein, a biomarker of wound chronicity, has been developed using nanoporous anodic alumina (NAA) membranes modified with Flii antibody recognition sites. The electrochemical detection is based on the nanochannel blockage experienced upon Flii capture by immobilized antibodies within the nanochannels. This capture impedes the diffusion of redox species [[Fe(CN)6]4−/3−] toward a gold electrode attached at the backside of the modified NAA membrane. Partial blockage causes a decrease in the oxidation current of the redox species at the electrode surface which is used as an analytical signal by the reported biosensor. The resulting biosensing system allows detection of Flii at the levels found in wounds. Two types of assays were tested, sandwich and direct, showing <3 and 2 h analysis time, respectively, a significant reduction in time from the nearly 48 h required for the conventional Western blot assay. Slightly higher sensitivity values were observed for the sandwich-based strategy. With faster analysis, lack of matrix effects, robustness, ease of use and cost-effectiveness, the developed sensing platform has the potential to be translated into a point-of-care (POC) device for chronic wound management and as a simple alternative characterization tool in Flii research.
  • Altres:

    Autor segons l'article: Rajeev G; Melville E; Cowin AJ; Prieto-Simon B; Voelcker NH
    Departament: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/s de la URV: Prieto Simón, Beatriz
    Paraules clau: Repair Porous alumina Nanohole arrays Immunosensing Flii Flightless-i Flightless detection Electrochemical biosensor Chronic wound Attenuation Actin-remodeling protein
    Resum: © Copyright © 2020 Rajeev, Melville, Cowin, Prieto-Simon and Voelcker. A label-free electrochemical detection platform for the sensitive and rapid detection of Flightless I (Flii) protein, a biomarker of wound chronicity, has been developed using nanoporous anodic alumina (NAA) membranes modified with Flii antibody recognition sites. The electrochemical detection is based on the nanochannel blockage experienced upon Flii capture by immobilized antibodies within the nanochannels. This capture impedes the diffusion of redox species [[Fe(CN)6]4−/3−] toward a gold electrode attached at the backside of the modified NAA membrane. Partial blockage causes a decrease in the oxidation current of the redox species at the electrode surface which is used as an analytical signal by the reported biosensor. The resulting biosensing system allows detection of Flii at the levels found in wounds. Two types of assays were tested, sandwich and direct, showing <3 and 2 h analysis time, respectively, a significant reduction in time from the nearly 48 h required for the conventional Western blot assay. Slightly higher sensitivity values were observed for the sandwich-based strategy. With faster analysis, lack of matrix effects, robustness, ease of use and cost-effectiveness, the developed sensing platform has the potential to be translated into a point-of-care (POC) device for chronic wound management and as a simple alternative characterization tool in Flii research.
    Àrees temàtiques: Química Medicina ii Medicina i Materiais Interdisciplinar General chemistry Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: beatriz.prieto-simon@urv.cat
    Identificador de l'autor: 0000-0001-8016-1565
    Data d'alta del registre: 2023-02-19
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.frontiersin.org/articles/10.3389/fchem.2020.00155/full
    Referència a l'article segons font original: Frontiers In Chemistry. 8 (155):
    Referència de l'ítem segons les normes APA: Rajeev G; Melville E; Cowin AJ; Prieto-Simon B; Voelcker NH (2020). Porous Alumina Membrane-Based Electrochemical Biosensor for Protein Biomarker Detection in Chronic Wounds. Frontiers In Chemistry, 8(155), -. DOI: 10.3389/fchem.2020.00155
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.3389/fchem.2020.00155
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2020
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Chemistry (Miscellaneous),Chemistry, Multidisciplinary
    Repair
    Porous alumina
    Nanohole arrays
    Immunosensing
    Flii
    Flightless-i
    Flightless detection
    Electrochemical biosensor
    Chronic wound
    Attenuation
    Actin-remodeling protein
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General chemistry
    Farmacia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
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