Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Porous Alumina Membrane-Based Electrochemical Biosensor for Protein Biomarker Detection in Chronic Wounds

  • Datos identificativos

    Identificador: imarina:6232132
    Autores:
    Rajeev GMelville ECowin AJPrieto-Simon BVoelcker NH
    Resumen:
    © Copyright © 2020 Rajeev, Melville, Cowin, Prieto-Simon and Voelcker. A label-free electrochemical detection platform for the sensitive and rapid detection of Flightless I (Flii) protein, a biomarker of wound chronicity, has been developed using nanoporous anodic alumina (NAA) membranes modified with Flii antibody recognition sites. The electrochemical detection is based on the nanochannel blockage experienced upon Flii capture by immobilized antibodies within the nanochannels. This capture impedes the diffusion of redox species [[Fe(CN)6]4−/3−] toward a gold electrode attached at the backside of the modified NAA membrane. Partial blockage causes a decrease in the oxidation current of the redox species at the electrode surface which is used as an analytical signal by the reported biosensor. The resulting biosensing system allows detection of Flii at the levels found in wounds. Two types of assays were tested, sandwich and direct, showing <3 and 2 h analysis time, respectively, a significant reduction in time from the nearly 48 h required for the conventional Western blot assay. Slightly higher sensitivity values were observed for the sandwich-based strategy. With faster analysis, lack of matrix effects, robustness, ease of use and cost-effectiveness, the developed sensing platform has the potential to be translated into a point-of-care (POC) device for chronic wound management and as a simple alternative characterization tool in Flii research.
  • Otros:

    Autor según el artículo: Rajeev G; Melville E; Cowin AJ; Prieto-Simon B; Voelcker NH
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Prieto Simón, Beatriz
    Palabras clave: Repair Porous alumina Nanohole arrays Immunosensing Flii Flightless-i Flightless detection Electrochemical biosensor Chronic wound Attenuation Actin-remodeling protein
    Resumen: © Copyright © 2020 Rajeev, Melville, Cowin, Prieto-Simon and Voelcker. A label-free electrochemical detection platform for the sensitive and rapid detection of Flightless I (Flii) protein, a biomarker of wound chronicity, has been developed using nanoporous anodic alumina (NAA) membranes modified with Flii antibody recognition sites. The electrochemical detection is based on the nanochannel blockage experienced upon Flii capture by immobilized antibodies within the nanochannels. This capture impedes the diffusion of redox species [[Fe(CN)6]4−/3−] toward a gold electrode attached at the backside of the modified NAA membrane. Partial blockage causes a decrease in the oxidation current of the redox species at the electrode surface which is used as an analytical signal by the reported biosensor. The resulting biosensing system allows detection of Flii at the levels found in wounds. Two types of assays were tested, sandwich and direct, showing <3 and 2 h analysis time, respectively, a significant reduction in time from the nearly 48 h required for the conventional Western blot assay. Slightly higher sensitivity values were observed for the sandwich-based strategy. With faster analysis, lack of matrix effects, robustness, ease of use and cost-effectiveness, the developed sensing platform has the potential to be translated into a point-of-care (POC) device for chronic wound management and as a simple alternative characterization tool in Flii research.
    Áreas temáticas: Química Medicina ii Medicina i Materiais Interdisciplinar General chemistry Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: beatriz.prieto-simon@urv.cat
    Identificador del autor: 0000-0001-8016-1565
    Fecha de alta del registro: 2023-02-19
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.frontiersin.org/articles/10.3389/fchem.2020.00155/full
    Referencia al articulo segun fuente origial: Frontiers In Chemistry. 8 (155):
    Referencia de l'ítem segons les normes APA: Rajeev G; Melville E; Cowin AJ; Prieto-Simon B; Voelcker NH (2020). Porous Alumina Membrane-Based Electrochemical Biosensor for Protein Biomarker Detection in Chronic Wounds. Frontiers In Chemistry, 8(155), -. DOI: 10.3389/fchem.2020.00155
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.3389/fchem.2020.00155
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Chemistry (Miscellaneous),Chemistry, Multidisciplinary
    Repair
    Porous alumina
    Nanohole arrays
    Immunosensing
    Flii
    Flightless-i
    Flightless detection
    Electrochemical biosensor
    Chronic wound
    Attenuation
    Actin-remodeling protein
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General chemistry
    Farmacia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
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