Autor segons l'article: Ortiz, Mayreli; Joda, Hamdi; Hoeth, Julian; Beni, Valerio; Katakis, Ioanis; Klemm, Richard; Lind, Kristina; O'Sullivan, Ciara K; Fragoso, Alex
Departament: Enginyeria Química
Autor/s de la URV: BENI, VALERIO / Fragoso Sierra, Alex / JODA, HAMDI ABDELAZIM OSMAN / Katakis, Ioanis / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli
Paraules clau: Lab-on-a-chip devices; Lab-on-a-chip; Integrated microsystem; Immunosensor; Immunoassay; Humans; Genosensor; Genetic techniques; Equipment design; Electrochemical techniques; Celiac disease; Biomarkers; Automated bioassay; Autoantibodies; integrated microsystem; immunosensor; genosensor; celiac disease; automated bioassay
Resum: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Celiac disease is an auto-immune disorder induced by ingestion of gluten in genetically predisposed individuals. Its diagnostics is more accurate using a combination of immunologic and genetic tests to detect of high levels of certain auto-antibodies and the presence human leukocyte antigen HLA-DQ2 or HLA-DQ8 genetic markers. In this work, we report the design and testing of automated microsystems combining sample treatment, storage, fluidic transport, and detection in a single platform able to carry out genetic or serologic analysis for detection of celiac disease markers. These microsystems share a common footprint and many fluidic features and are thus able to perform a complete assay. The microsystem for the genetic assay extracts and amplifies the DNA prior to detection, while the serology microsystem contains a filter and chamber for the generation and subsequent dilution of plasma. The performance of both platforms is demonstrated and compared with reference methods with an excellent correlation, which makes the developed platform amenable for clinical studies.
Àrees temàtiques: Zootecnia / recursos pesqueiros; Química; Medicina ii; Medicina i; Materiais; Interdisciplinar; General medicine; Farmacia; Clinical biochemistry; Ciências biológicas ii; Ciências biológicas i; Ciências agrárias i; Ciência de alimentos; Chemistry, analytical; Biotecnología; Biodiversidade; Biochemistry; Biochemical research methods; Analytical chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: mayreli.ortiz@urv.cat; mayreli.ortiz@urv.cat; ioanis.katakis@urv.cat; alex.fragoso@urv.cat
ISSN: 01730835
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201500140
Referència a l'article segons font original: Electrophoresis. 36 (16): 1920-1926
Referència de l'ítem segons les normes APA: Ortiz, Mayreli; Joda, Hamdi; Hoeth, Julian; Beni, Valerio; Katakis, Ioanis; Klemm, Richard; Lind, Kristina; O'Sullivan, Ciara K; Fragoso, Alex (2015). Bleed-to-read disposable microsystems for the genetic and serological analysis of celiac disease markers with amperometric detection. Electrophoresis, 36(16), 1920-1926. DOI: 10.1002/elps.201500140
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1002/elps.201500140
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2015
Tipus de publicació: Journal Publications
Repositori URV