Bleed-to-read disposable microsystems for the genetic and serological analysis of celiac disease markers with amperometric detection

Identifier:  imarina:6388297

Handle: https://hdl.handle.net/20.500.11797/imarina6388297

Authors:  Ortiz, M; Joda, H; Höth, J; Beni, V; Katakis, I; Klemm, R; Lind, K; O'Sullivan, CK; Fragoso, A

Abstract:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Celiac disease is an auto-immune disorder induced by ingestion of gluten in genetically predisposed individuals. Its diagnostics is more accurate using a combination of immunologic and genetic tests to detect of high levels of certain auto-antibodies and the presence human leukocyte antigen HLA-DQ2 or HLA-DQ8 genetic markers. In this work, we report the design and testing of automated microsystems combining sample treatment, storage, fluidic transport, and detection in a single platform able to carry out genetic or serologic analysis for detection of celiac disease markers. These microsystems share a common footprint and many fluidic features and are thus able to perform a complete assay. The microsystem for the genetic assay extracts and amplifies the DNA prior to detection, while the serology microsystem contains a filter and chamber for the generation and subsequent dilution of plasma. The performance of both platforms is demonstrated and compared with reference methods with an excellent correlation, which makes the developed platform amenable for clinical studies.