Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Metabolomics reveals novel blood plasma biomarkers associated to the BRCA1-mutated phenotype of human breast cancer

  • Dades identificatives

    Identificador: imarina:6389321
    Autors:
    Roig, BarbaraRodriguez-Balada, MartaSamino, SaraLam, Eric W -FGuaita-Esteruelas, SandraGomes, Ana RCorreig, XavierBorras, JoanYanes, OscarGuma, Josep
    Resum:
    © 2017 The Author(s). Hereditary breast and ovarian cancer syndrome (HBOC) is partly due to the presence of mutations in the BRCA genes. Triple-negative (TN) breast cancer (BC) shares histological characteristics with germline BRCA1 mutation-associated tumours. We have investigated the metabolic profiles of human breast cancer (BC) cell lines carrying BRCA1 pathogenic mutations by non-targeted liquid chromatography coupled to mass spectrometry technology. Based on our in vitro results, we performed a targeted metabolomic analysis of plasma samples from TN HBOC patients taking into account their BRCA1 genotype. BRCA1 promoter hypermethylation and the BRCAness phenotype of BC cell lines were also studied. The purpose of this study was to determine the metabolic signature of HBOC syndrome and TNBC patients and to evaluate the potential contribution of the metabolites identified to the genetic diagnosis of breast cancer. The present results show the existence of a differential metabolic signature for BC cells based on the BRCA1 functionality. None of the studied BC cell lines presented hypermethylation of the BRCA1 promoter region. We provide evidence of the existence of free methylated nucleotides capable of distinguishing plasma samples from HBOC patients as BRCA1-mutated and BRCA1 non-mutated, suggesting that they might be considered as BRCA1-like biomarkers for TNBC and HBOC syndrome.
  • Altres:

    Autor segons l'article: Roig, Barbara; Rodriguez-Balada, Marta; Samino, Sara; Lam, Eric W -F; Guaita-Esteruelas, Sandra; Gomes, Ana R; Correig, Xavier; Borras, Joan; Yanes, Oscar; Guma, Josep
    Departament: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/s de la URV: BORRAS BALADA, JOAN LLUÍS / Correig Blanchar, Francesc Xavier / GUAITA ESTERUELAS, SANDRA / Gumà Padró, José / RODRÍGUEZ BALADA, MARTA / Roig Bourgine, Barbara / SAMINO GENÉ, SARA / Yanes Torrado, Óscar
    Paraules clau: Triple negative breast neoplasms Promoter regions, genetic Phenotype Middle aged Metabolomics Metabolome Mcf-7 cells Humans Hereditary breast and ovarian cancer syndrome Germ-line mutation Female Dna methylation Cell line, tumor Brca1 protein, human Brca1 protein Biomarkers, tumor
    Resum: © 2017 The Author(s). Hereditary breast and ovarian cancer syndrome (HBOC) is partly due to the presence of mutations in the BRCA genes. Triple-negative (TN) breast cancer (BC) shares histological characteristics with germline BRCA1 mutation-associated tumours. We have investigated the metabolic profiles of human breast cancer (BC) cell lines carrying BRCA1 pathogenic mutations by non-targeted liquid chromatography coupled to mass spectrometry technology. Based on our in vitro results, we performed a targeted metabolomic analysis of plasma samples from TN HBOC patients taking into account their BRCA1 genotype. BRCA1 promoter hypermethylation and the BRCAness phenotype of BC cell lines were also studied. The purpose of this study was to determine the metabolic signature of HBOC syndrome and TNBC patients and to evaluate the potential contribution of the metabolites identified to the genetic diagnosis of breast cancer. The present results show the existence of a differential metabolic signature for BC cells based on the BRCA1 functionality. None of the studied BC cell lines presented hypermethylation of the BRCA1 promoter region. We provide evidence of the existence of free methylated nucleotides capable of distinguishing plasma samples from HBOC patients as BRCA1-mutated and BRCA1 non-mutated, suggesting that they might be considered as BRCA1-like biomarkers for TNBC and HBOC syndrome.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20452322
    Adreça de correu electrònic de l'autor: oscar.yanes@urv.cat barbara.roig@urv.cat jose.guma@urv.cat xavier.correig@urv.cat
    Identificador de l'autor: 0000-0003-3695-7157 0000-0001-7541-9832 0000-0002-6902-3054
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.nature.com/articles/s41598-017-17897-8
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Scientific Reports. 7 (1): 17831-
    Referència de l'ítem segons les normes APA: Roig, Barbara; Rodriguez-Balada, Marta; Samino, Sara; Lam, Eric W -F; Guaita-Esteruelas, Sandra; Gomes, Ana R; Correig, Xavier; Borras, Joan; Yanes, O (2017). Metabolomics reveals novel blood plasma biomarkers associated to the BRCA1-mutated phenotype of human breast cancer. Scientific Reports, 7(1), 17831-. DOI: 10.1038/s41598-017-17897-8
    DOI de l'article: 10.1038/s41598-017-17897-8
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2017
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Multidisciplinary,Multidisciplinary Sciences
    Triple negative breast neoplasms
    Promoter regions, genetic
    Phenotype
    Middle aged
    Metabolomics
    Metabolome
    Mcf-7 cells
    Humans
    Hereditary breast and ovarian cancer syndrome
    Germ-line mutation
    Female
    Dna methylation
    Cell line, tumor
    Brca1 protein, human
    Brca1 protein
    Biomarkers, tumor
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Odontología
    Nutrição
    Multidisciplinary sciences
    Multidisciplinary
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Letras / linguística
    Interdisciplinar
    Geografía
    Geociências
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Educação física
    Educação
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
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