Autor según el artículo: Roig B; Rodríguez-Balada M; Samino S; Lam E; Guaita-Esteruelas S; Gomes A; Correig X; Borràs J; Yanes O; Gumà J

Departamento: Enginyeria Electrònica, Elèctrica i Automàtica

Autor/es de la URV: BORRAS BALADA, JOAN LLUÍS / Correig Blanchar, Francesc Xavier / GUAITA ESTERUELAS, SANDRA / Gumà Padró, José / RODRÍGUEZ BALADA, MARTA / SAMINO GENÉ, SARA / Yanes Torrado, Óscar

Resumen: © 2017 The Author(s). Hereditary breast and ovarian cancer syndrome (HBOC) is partly due to the presence of mutations in the BRCA genes. Triple-negative (TN) breast cancer (BC) shares histological characteristics with germline BRCA1 mutation-associated tumours. We have investigated the metabolic profiles of human breast cancer (BC) cell lines carrying BRCA1 pathogenic mutations by non-targeted liquid chromatography coupled to mass spectrometry technology. Based on our in vitro results, we performed a targeted metabolomic analysis of plasma samples from TN HBOC patients taking into account their BRCA1 genotype. BRCA1 promoter hypermethylation and the BRCAness phenotype of BC cell lines were also studied. The purpose of this study was to determine the metabolic signature of HBOC syndrome and TNBC patients and to evaluate the potential contribution of the metabolites identified to the genetic diagnosis of breast cancer. The present results show the existence of a differential metabolic signature for BC cells based on the BRCA1 functionality. None of the studied BC cell lines presented hypermethylation of the BRCA1 promoter region. We provide evidence of the existence of free methylated nucleotides capable of distinguishing plasma samples from HBOC patients as BRCA1-mutated and BRCA1 non-mutated, suggesting that they might be considered as BRCA1-like biomarkers for TNBC and HBOC syndrome.

Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20452322

Direcció de correo del autor: xavier.correig@urv.cat oscar.yanes@urv.cat jose.guma@urv.cat

Identificador del autor: 0000-0002-6902-3054 0000-0003-3695-7157 0000-0001-7541-9832

Fecha de alta del registro: 2023-02-19

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.nature.com/articles/s41598-017-17897-8

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Scientific Reports. 7 (1): 17831-

Referencia de l'ítem segons les normes APA: Roig B; Rodríguez-Balada M; Samino S; Lam E; Guaita-Esteruelas S; Gomes A; Correig X; Borràs J; Yanes O; Gumà J (2017). Metabolomics reveals novel blood plasma biomarkers associated to the BRCA1-mutated phenotype of human breast cancer. Scientific Reports, 7(1), 17831-. DOI: 10.1038/s41598-017-17897-8

DOI del artículo: 10.1038/s41598-017-17897-8

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Tipo de publicación: Journal Publications