Surface-Enhanced Raman Scattering Detection of Nucleic Acids Exhibiting Sterically Accessible Guanines Using Ruthenium-Polypyridyl Reagents

Identificador:  imarina:8263152

Handle: https://hdl.handle.net/20.500.11797/imarina8263152

Autors:  Martinez-Calvo, Miguel; Guerrini, Luca; Rodriguez, Jessica; Alvarez-Puebla, Ramon A; Mascarenas, Jose L

Resum:
Here, we report the application of surface-enhanced Raman scattering (SERS) spectroscopy as a rapid and practical tool for assessing the formation of coordinative adducts between nucleic acid guanines and ruthenium polypyridyl reagents. The technology provides a practical approach for the wash-free and quick identification of nucleic acid structures exhibiting sterically accessible guanines. This is demonstrated for the detection of a quadruplex-forming sequence present in the promoter region of the c-myc oncogene, which exhibits a nonpaired, reactive guanine at a flanking position of the G-quartets.