Autor segons l'article: Valente, Sabrina; Ciavarella, Carmen; Hernandez-Aguilera, Anna; Salvador, Fernandez-Arroyo; Buzzi, Marina; Joven, Jorge; Pasquinelli, Gianandrea;
Departament: Medicina i Cirurgia
Autor/s de la URV: Joven Maried, Jorge
Paraules clau: Stromal cells Stem cell metabolomics Spheroids Spheres Pathways Human mesenchymal stem cells Culture Arteries
Resum: The ability to form spheroids under non-adherent conditions is a well-known property of human mesenchymal stem cells (hMSCs), in addition to stemness and multilineage differentiation features. In the present study, we tested the ability of hMSCs isolated from the vascular wall (hVW-MSCs) to grow as spheres, and provide a characterization of this 3D model. hVW-MSCs were isolated from femoral arteries through enzymatic digestion. Spheres were obtained using ultra-low attachment and hanging drop methods. Immunophenotype and pluripotent genes (SOX-2, OCT-4, NANOG) were analyzed by immunocytochemistry and real-time PCR, respectively. Spheres histological and ultrastructural architecture were examined. Cell viability and proliferative capacity were measured using LIVE/DEATH assay and ki-67 proliferation marker. Metabolomic profile was obtained with liquid chromatography-mass spectrometry. In 2D, hVW-MSCs were spindle-shaped, expressed mesenchymal antigens, and displayed mesengenic potential. 3D cultures of hVW-MSCs were CD44(+), CD105(low), CD90(low), exhibited a low propensity to enter the cell cycle as indicated by low percentage of ki-67 expression and accumulated intermediate metabolites pointing to slowed metabolism. The 3D model of hVW-MSCs exhibits stemness, dormancy and slow metabolism, typically observed in stem cell niches. This culture strategy can represent an accurate model to investigate hMSCs features for future clinical applications in the vascular field.
Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Microscopy Medicina veterinaria Medicina iii Medicina ii Medicina i Medical laboratory technology Materiais Matemática / probabilidade e estatística Interdisciplinar Instrumentation Histology Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biology Biodiversidade Astronomia / física Anatomy & morphology Anatomy
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: jorge.joven@urv.cat
Identificador de l'autor: 0000-0003-2749-4541
Data d'alta del registre: 2024-08-31
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.23918
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Microscopy Research And Technique. 85 (2): 447-459
Referència de l'ítem segons les normes APA: Valente, Sabrina; Ciavarella, Carmen; Hernandez-Aguilera, Anna; Salvador, Fernandez-Arroyo; Buzzi, Marina; Joven, Jorge; Pasquinelli, Gianandrea; (2022). Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells. Microscopy Research And Technique, 85(2), 447-459. DOI: 10.1002/jemt.23918
DOI de l'article: 10.1002/jemt.23918
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV