Autor segons l'article: Pascual-Caro, Sergi; Borrull, Francesc; Aguilar, Carme; Calull, Marta;
Departament: Química Analítica i Química Orgànica
Autor/s de la URV: Aguilar Anguera, Maria del Carmen / Borrull Ballarín, Francesc / Calull Blanch, Marta / Pascual Caro, Sergi
Paraules clau: Stimulants Separation Ms Methamphetamine Metabolites Lisdexamfetamine Liquid-chromatography Human urine Hair Derivatization Derivatives Chiral selectors Ce-ms/ms Ce-ms Cation-exchange sorbents Amphetamine-type substances
Resum: The present study develops a method for the enantioseparation of a group of amphetamines and their metabolites in urine by CE coupled to MS/MS (CE-MS/MS). Amphetamines present a chiral center and thus two enantiomers, which is important from a toxicological point of view because they may have different pharmacokinetic and pharmacological properties. It is therefore essential to find suitable methods to distinguish both enantiomers. Today the use of CE is becoming more important in this field since, with the simple addition of a chiral selector to the background electrolyte, the enantioseparation can easily be achieved. However, when CE is coupled to MS, the use of volatile chiral selectors and compatible background electrolytes or other strategies such as the countercurrent migration approach are required to avoid contamination of the ion source from nonvolatile species. In the present study, we use the latter strategy to evaluate six different chiral selectors using CE-MS/MS. As a sample pre-treatment, two cationic-exchange sorbents-Oasis WCX and Oasis MCX-are compared for the urine pre-treatment. Using this method, it was possible to achieve the complete chiral separation of the amphetamines under study with detection limits ranging between 0.8 and 1.5 ng/mL and method quantification limits between 2.0 and 8.0 ng/mL. Matrix-matched calibration curves up to 150 ng/mL were used to cover the usual concentration ranges at which amphetamines have generally been found in toxicological and forensic analyses.
Àrees temàtiques: Zootecnia / recursos pesqueiros Química Medicina ii Medicina i Materiais Interdisciplinar General medicine Farmacia Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, analytical Biotecnología Biodiversidade Biochemistry Biochemical research methods Analytical chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: francesc.borrull@urv.cat marta.calull@urv.cat carme.aguilar@urv.cat sergi.pascual@estudiants.urv.cat
Identificador de l'autor: 0000-0003-2718-9336 0000-0002-5258-1949 0000-0002-5913-2599 0000-0001-8890-5100
Data d'alta del registre: 2023-02-23
Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
Enllaç font original: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100231
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Electrophoresis. 43 (3): 437-445
Referència de l'ítem segons les normes APA: Pascual-Caro, Sergi; Borrull, Francesc; Aguilar, Carme; Calull, Marta; (2022). Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry. Electrophoresis, 43(3), 437-445. DOI: 10.1002/elps.202100231
DOI de l'article: 10.1002/elps.202100231
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV