Autor segons l'article: Itarte M; Bofill-Mas S; Martínez-Puchol S; Torrell H; Ceretó A; Carrasco M; Forés E; Canela N; Girones R; Rusiñol M
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Cereto Massagué, Adrián José
Paraules clau: Wastewater-based epidemiology (wbe) Variants of interest (vois) Variants of concern (vocs) Signature mutations Sars-cov-2 Next-generation sequencing (ngs)
Resum: SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective. © 2021 The Authors
Àrees temàtiques: Public health, environmental and occupational health Health, toxicology and mutagenesis Environmental chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: adrianjose.cereto@urv.cat
Data d'alta del registre: 2024-07-27
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/pii/S2468584421000805?via%3Dihub
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Current Opinion In Environmental Science And Health. 24
Referència de l'ítem segons les normes APA: Itarte M; Bofill-Mas S; Martínez-Puchol S; Torrell H; Ceretó A; Carrasco M; Forés E; Canela N; Girones R; Rusiñol M (2021). Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage. Current Opinion In Environmental Science And Health, 24(), -. DOI: 10.1016/j.coesh.2021.100308
DOI de l'article: 10.1016/j.coesh.2021.100308
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2021
Tipus de publicació: Journal Publications
Repositori URV