Autor segons l'article: Calderon-Perez, Lorena; Suarez-Garcia, Susana; Pedret, Anna; Suarez, Manuel; Llaurado, Elisabet; Rubio, Laura; del Bas, Josep M; Caimari, Antoni; Puiggros, Francesc; Arola, Lluis; Sola, Rosa; Valls, Rosa M
Departament: Medicina i Cirurgia Bioquímica i Biotecnologia
Autor/s de la URV: Arola Ferrer, Luis Maria / Calderon Pérez, Lorena / Llauradó Ribé, Elisabet / Pedret Figuerola, Anna / Solà Alberich, Rosa Maria / SUÁREZ GARCÍA, SUSANA / Suárez Recio, Manuel / Valls Zamora, Rosa Maria
Paraules clau: Trimethylamine-n-oxide Polyunsaturated fatty acids Lysophospholipids Lysophosphatidylethanolamines Lysophosphatidylcholines Hypercholesterolemia
Resum: Background & aims: Whether bioactive lysophospholipids (lyso-PLs) and trimethylamine-N-oxide (TMAO) serve as non-invasive biomarkers in early human hypercholesterolemia (HC) is unknown. This study aimed to assess whether serum lyso-PLs and plasma TMAO may be suitable susceptibility/risk biomarkers of HC in humans. Secondarily, we aimed to evaluate the relationships between targeted metabolites, diet composition and circulating liver transaminases, and verify these results in hamsters. Methods: A targeted metabolomics and lipidomics approach determined plasma TMAO and serum lysophosphatidylcholines (lyso-PCs) and lysophosphatidylethanolamines (lyso-PEs) in low (L-LDL-c) and moderate to high (MH-LDL-c) LDL-cholesterol subjects. Additionally, the relationships between targeted metabolites, liver transaminases and diet, particularly fatty acid intake, were tested. In parallel, plasma and liver lyso-PL profiles were studied in 16 hamsters fed a moderate high-fat (HFD) or low-fat (LFD) diet for 30 days. Results: Predictive models identified lyso-PC15:0 and lyso-PE18:2 as the most discriminant lyso-PLs among groups. In MH-LDL-c (n = 48), LDL-cholesterol and saturated FAs were positively associated with lyso-PC15:0, whereas in L-LDL-c (n = 70), LDL-cholesterol and polyunsaturated fatty acids (PUFAs) were negatively and positively related to lyso-PE18:2, respectively. Interestingly, in MH-LDL-c, the lower lyso-PE 18:2 concentrations were indicative of higher LDL-cholesterol levels. Intrahepatic accumulation of lyso-PLs-containing essential n-6 PUFAs, including lyso-PE18:2, were higher in HFD-fed hamsters than LFD-fed hamsters. Conclusions: Overall, results revealed a possible hepatic adaptive mechanism to counteract diet-induced steatosis in animal and hypercholesterolemia progression in humans. In particular, low serum lyso-PE18:2 suggests a suitable susceptibility/risk biomarker of HC in humans.
Àrees temàtiques: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Educação física Critical care and intensive care medicine Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0261-5614
Adreça de correu electrònic de l'autor: lorena.calderon@urv.cat anna.pedret@urv.cat elisabet.llaurado@urv.cat manuel.suarez@urv.cat lorena.calderon@urv.cat rosamaria.valls@urv.cat rosa.sola@urv.cat lluis.arola@urv.cat
Identificador de l'autor: 0000-0003-0766-0733 0000-0002-5327-932X 0000-0002-7439-9531 0000-0003-0122-8253 0000-0003-0766-0733 0000-0002-3351-0942 0000-0002-8359-235X 0000-0003-2767-1974
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Clinical Nutrition. 41 (2): 489-499
Referència de l'ítem segons les normes APA: Calderon-Perez, Lorena; Suarez-Garcia, Susana; Pedret, Anna; Suarez, Manuel; Llaurado, Elisabet; Rubio, Laura; del Bas, Josep M; Caimari, Antoni; Puig (2022). Serum lysophospholipidome of dietary origin as a suitable susceptibility/risk biomarker of human hypercholesterolemia: A cross-sectional study. Clinical Nutrition, 41(2), 489-499. DOI: 10.1016/j.clnu.2021.11.033
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV