Autor segons l'article: Iakab SA; Baquer G; Lafuente M; Pina MP; Ramírez JL; Ràfols P; Correig-Blanchar X; García-Altares M
Departament: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/s de la URV: Baquer Gómez, Gerard Sergi / Correig Blanchar, Francesc Xavier / Garcia-Altares Pérez, Maria / Ràfols Soler, Pere / Ramírez Falo, José Luis
Paraules clau: Desorption/ionization mass-spectrometry workflow vibrational spectroscopy scattering raman-spectroscopy maldi-tof images database breast-cancer
Resum: Imaging techniques based on mass spectrometry or spectroscopy methods inform in situ about the chemical composition of biological tissues or organisms, but they are sometimes limited by their specificity, sensitivity, or spatial resolution. Multimodal imaging addresses these limitations by combining several imaging modalities; however, measuring the same sample with the same preparation using multiple imaging techniques is still uncommon due to the incompatibility between substrates, sample preparation protocols, and data formats. We present a multimodal imaging approach that employs a gold-coated nanostructured silicon substrate to couple surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and surface-enhanced Raman spectroscopy (SERS). Our approach integrates both imaging modalities by using the same substrate, sample preparation, and data analysis software on the same sample, allowing the coregistration of both images. We transferred molecules from clean fingertips and fingertips covered with plasticine modeling clay onto our nanostructure and analyzed their chemical composition and distribution by SALDI-MS and SERS. Multimodal analysis located the traces of plasticine on fingermarks and provided chemical information on the composition of the clay. Our multimodal approach effectively combines the advantages of mass spectrometry and vibrational spectroscopy with the signal enhancing abilities of our nanostructured substrate.
Àrees temàtiques: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: xavier.correig@urv.cat maria.garcia-altares@urv.cat pere.rafols@urv.cat joseluis.ramirez@urv.cat gerard.baquer@estudiants.urv.cat gerard.baquer@estudiants.urv.cat
Identificador de l'autor: 0000-0002-6902-3054 0000-0002-9240-4058 0000-0001-8231-4019 0000-0002-4433-4972 0000-0002-4433-4972
Data d'alta del registre: 2023-02-23
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://pubs.acs.org/doi/10.1021/acs.analchem.1c04118
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Analytical Chemistry. 94 (6): 2785-2793
Referència de l'ítem segons les normes APA: Iakab SA; Baquer G; Lafuente M; Pina MP; Ramírez JL; Ràfols P; Correig-Blanchar X; García-Altares M (2022). SALDI-MS and SERS Multimodal Imaging: One Nanostructured Substrate to Rule Them Both. Analytical Chemistry, 94(6), 2785-2793. DOI: 10.1021/acs.analchem.1c04118
DOI de l'article: 10.1021/acs.analchem.1c04118
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV