Autor segons l'article: Turino, Mariacristina; Alvarez-Puebla, Ramon A.; Guerrini, Luca;
Departament: Química Física i Inorgànica
Autor/s de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Turino, Mariacristina
Codi de projecte: Grant agreement No. 713679
Paraules clau: Surface-enhanced raman scattering Silver nanoparticles Sers quantification Plasmonics Nanoparticles Molecular-weight biothiols Metal Low molecular weight thiols Glutathione Biosensing
Resum: Low molecular weight thiols (biothiols) are highly active compounds extensively involved in human physiology. Their abnormal levels have been associated with multiple diseases. In recent years, major efforts have been devoted to developing new nanosensing methods for the low cost and fast quantification of this class of analytes in minimally pre-treated samples. Herein, we present a novel strategy for engineering a highly efficient surface-enhanced Raman scattering (SERS) spectroscopy platform for the dynamic sensing of biothiols. Colloidally stable silver nanoparticles clusters equipped with a specifically designed azobenzene derivative (AzoProbe) were generated as highly SERS active substrates. In the presence of small biothiols (e.g., glutathione, GSH), breakage of the AzoProbe diazo bond causes drastic spectral changes that can be quantitatively correlated with the biothiol content with a limit of detection of ca. 5 nM for GSH. An identical response was observed for other low molecular weight thiols, while larger macromolecules with free thiol groups (e.g., bovine serum albumin) do not produce distinguishable spectral alterations. This indicates the suitability of the SERS sensing platform for the selective quantification of small biothiols.
Àrees temàtiques: Química Nanoscience & nanotechnology Medicine (miscellaneous) Materiais Instruments & instrumentation Instrumentation Engineering (miscellaneous) Clinical biochemistry Ciencias sociales Ciências biológicas iii Ciências biológicas i Chemistry, analytical Biotecnología Biotechnology Biomedical engineering Astronomia / física Analytical chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: ramon.alvarez@urv.cat mariacristina.turino@estudiants.urv.cat luca.guerrini@urv.cat
Identificador de l'autor: 0000-0003-4770-5756 0000-0003-3053-4970 0000-0002-2925-1562
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.mdpi.com/2079-6374/12/5/267
Programa de finançament: Marie Skłodowska-Curie Actions - European Union's Horizon 2020 research and innovation programme
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Biosensors. 12 (5):
Referència de l'ítem segons les normes APA: Turino, Mariacristina; Alvarez-Puebla, Ramon A.; Guerrini, Luca; (2022). Plasmonic Azobenzene Chemoreporter for Surface-Enhanced Raman Scattering Detection of Biothiols. Biosensors, 12(5), -. DOI: 10.3390/bios12050267
Acrònim: MFP
DOI de l'article: 10.3390/bios12050267
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Acció del programa de finançament: Martí i Franquès COFUND Doctoral Programme
Tipus de publicació: Journal Publications