Articles producció científica> Química Física i Inorgànica

Plasmonic Azobenzene Chemoreporter for Surface-Enhanced Raman Scattering Detection of Biothiols

  • Datos identificativos

    Identificador: imarina:9265252
    Autores:
    Turino, MariacristinaAlvarez-Puebla, Ramon A.Guerrini, Luca
    Resumen:
    Low molecular weight thiols (biothiols) are highly active compounds extensively involved in human physiology. Their abnormal levels have been associated with multiple diseases. In recent years, major efforts have been devoted to developing new nanosensing methods for the low cost and fast quantification of this class of analytes in minimally pre-treated samples. Herein, we present a novel strategy for engineering a highly efficient surface-enhanced Raman scattering (SERS) spectroscopy platform for the dynamic sensing of biothiols. Colloidally stable silver nanoparticles clusters equipped with a specifically designed azobenzene derivative (AzoProbe) were generated as highly SERS active substrates. In the presence of small biothiols (e.g., glutathione, GSH), breakage of the AzoProbe diazo bond causes drastic spectral changes that can be quantitatively correlated with the biothiol content with a limit of detection of ca. 5 nM for GSH. An identical response was observed for other low molecular weight thiols, while larger macromolecules with free thiol groups (e.g., bovine serum albumin) do not produce distinguishable spectral alterations. This indicates the suitability of the SERS sensing platform for the selective quantification of small biothiols.
  • Otros:

    Autor según el artículo: Turino, Mariacristina; Alvarez-Puebla, Ramon A.; Guerrini, Luca;
    Departamento: Química Física i Inorgànica
    Autor/es de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Turino, Mariacristina
    Código de proyecto: Grant agreement No. 713679
    Palabras clave: Surface-enhanced raman scattering Silver nanoparticles Sers quantification Plasmonics Nanoparticles Molecular-weight biothiols Metal Low molecular weight thiols Glutathione Biosensing
    Resumen: Low molecular weight thiols (biothiols) are highly active compounds extensively involved in human physiology. Their abnormal levels have been associated with multiple diseases. In recent years, major efforts have been devoted to developing new nanosensing methods for the low cost and fast quantification of this class of analytes in minimally pre-treated samples. Herein, we present a novel strategy for engineering a highly efficient surface-enhanced Raman scattering (SERS) spectroscopy platform for the dynamic sensing of biothiols. Colloidally stable silver nanoparticles clusters equipped with a specifically designed azobenzene derivative (AzoProbe) were generated as highly SERS active substrates. In the presence of small biothiols (e.g., glutathione, GSH), breakage of the AzoProbe diazo bond causes drastic spectral changes that can be quantitatively correlated with the biothiol content with a limit of detection of ca. 5 nM for GSH. An identical response was observed for other low molecular weight thiols, while larger macromolecules with free thiol groups (e.g., bovine serum albumin) do not produce distinguishable spectral alterations. This indicates the suitability of the SERS sensing platform for the selective quantification of small biothiols.
    Áreas temáticas: Química Nanoscience & nanotechnology Medicine (miscellaneous) Materiais Instruments & instrumentation Instrumentation Engineering (miscellaneous) Clinical biochemistry Ciencias sociales Ciências biológicas iii Ciências biológicas i Chemistry, analytical Biotecnología Biotechnology Biomedical engineering Astronomia / física Analytical chemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: ramon.alvarez@urv.cat mariacristina.turino@estudiants.urv.cat luca.guerrini@urv.cat
    Identificador del autor: 0000-0003-4770-5756 0000-0003-3053-4970 0000-0002-2925-1562
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2079-6374/12/5/267
    Programa de financiación: Marie Skłodowska-Curie Actions - European Union's Horizon 2020 research and innovation programme
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Biosensors. 12 (5):
    Referencia de l'ítem segons les normes APA: Turino, Mariacristina; Alvarez-Puebla, Ramon A.; Guerrini, Luca; (2022). Plasmonic Azobenzene Chemoreporter for Surface-Enhanced Raman Scattering Detection of Biothiols. Biosensors, 12(5), -. DOI: 10.3390/bios12050267
    Acrónimo: MFP
    DOI del artículo: 10.3390/bios12050267
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Acción del progama de financiación: Martí i Franquès COFUND Doctoral Programme
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Analytical Chemistry,Biomedical Engineering,Biotechnology,Chemistry, Analytical,Clinical Biochemistry,Instrumentation,Instruments & Instrumentation,Medicine (Miscellaneous),Nanoscience & Nanotechnology
    Surface-enhanced raman scattering
    Silver nanoparticles
    Sers quantification
    Plasmonics
    Nanoparticles
    Molecular-weight biothiols
    Metal
    Low molecular weight thiols
    Glutathione
    Biosensing
    Química
    Nanoscience & nanotechnology
    Medicine (miscellaneous)
    Materiais
    Instruments & instrumentation
    Instrumentation
    Engineering (miscellaneous)
    Clinical biochemistry
    Ciencias sociales
    Ciências biológicas iii
    Ciências biológicas i
    Chemistry, analytical
    Biotecnología
    Biotechnology
    Biomedical engineering
    Astronomia / física
    Analytical chemistry
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