Autor segons l'article: Haeusler, Rebecca A.; Camastra, Stefania; Astiarraga, Brenno; Nannipieri, Monica; Anselmino, Marco; Ferrannini, Ele;
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Domínguez Porfirio, Brenno
Paraules clau: Postprandial hyperglycemia Metabolism Liver Humans Glucose-production Gluconeogenesis Glucokinase Gene-expression Foxo1 Fatty rats Fasting hyperglycemia Diabetes De-novo lipogenesis
Resum: Background/objectives: Increased endogenous glucose production is a hallmark of type 2 diabetes. Evidence from animal models has suggested that a likely cause of this is increased mRNA expression of glucose 6-phosphatase and phosphoenolpyruvate carboxykinase (encoded by G6PC, PCK1 and PCK2). But another contributing factor may be decreased liver glucokinase (encoded by GCK).Methods: We examined expression of these enzymes in liver biopsies from 12 nondiabetic and 28 diabetic individuals. Diabetic patients were further separated into those with HbA1c lower or higher than 7.0.Results: In diabetic subjects with HbA1c > 7.0, we found that gluconeogenic enzymes were expressed normally, but GCK was suppressed more than 60%. Moreover, HbA1c and fasting glucose were negatively correlated with GCK, but showed no correlation with G6PC, PCK1, or PCK2.Conclusion: These findings suggest an underlying dysregulation of hepatic GCK expression during frank diabetes, which has implications for the therapeutic use of glucokinase activators in this population. (C) 2014 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Àrees temàtiques: Molecular biology Endocrinology & metabolism Ciências biológicas ii Cell biology
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: brenno.dominguez@urv.cat
Identificador de l'autor: 0000-0003-2216-8974
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/pii/S2212877814002087?via%3Dihub
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Molecular Metabolism. 4 (3): 222-226
Referència de l'ítem segons les normes APA: Haeusler, Rebecca A.; Camastra, Stefania; Astiarraga, Brenno; Nannipieri, Monica; Anselmino, Marco; Ferrannini, Ele; (2015). Decreased expression of hepatic glucokinase in type 2 diabetes. Molecular Metabolism, 4(3), 222-226. DOI: 10.1016/j.molmet.2014.12.007
DOI de l'article: 10.1016/j.molmet.2014.12.007
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2015
Tipus de publicació: Journal Publications
Repositori URV