Articles producció científica> Bioquímica i Biotecnologia

Decreased expression of hepatic glucokinase in type 2 diabetes

  • Identification data

    Identifier: imarina:9293243
  • Authors:

    Haeusler, Rebecca A.
    Camastra, Stefania
    Astiarraga, Brenno
    Nannipieri, Monica
    Anselmino, Marco
    Ferrannini, Ele
  • Others:

    Author, as appears in the article.: Haeusler, Rebecca A.; Camastra, Stefania; Astiarraga, Brenno; Nannipieri, Monica; Anselmino, Marco; Ferrannini, Ele;
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Domínguez Porfirio, Brenno
    Keywords: De-novo lipogenesis Diabetes Fasting hyperglycemia Fatty rats Foxo1 Gene-expression Glucokinase Gluconeogenesis Glucose-production Humans Liver Metabolism Postprandial hyperglycemia
    Abstract: Background/objectives: Increased endogenous glucose production is a hallmark of type 2 diabetes. Evidence from animal models has suggested that a likely cause of this is increased mRNA expression of glucose 6-phosphatase and phosphoenolpyruvate carboxykinase (encoded by G6PC, PCK1 and PCK2). But another contributing factor may be decreased liver glucokinase (encoded by GCK).Methods: We examined expression of these enzymes in liver biopsies from 12 nondiabetic and 28 diabetic individuals. Diabetic patients were further separated into those with HbA1c lower or higher than 7.0.Results: In diabetic subjects with HbA1c > 7.0, we found that gluconeogenic enzymes were expressed normally, but GCK was suppressed more than 60%. Moreover, HbA1c and fasting glucose were negatively correlated with GCK, but showed no correlation with G6PC, PCK1, or PCK2.Conclusion: These findings suggest an underlying dysregulation of hepatic GCK expression during frank diabetes, which has implications for the therapeutic use of glucokinase activators in this population. (C) 2014 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
    Thematic Areas: Cell biology Ciências biológicas ii Endocrinology & metabolism Molecular biology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: brenno.dominguez@urv.cat
    Author identifier: 0000-0003-2216-8974
    Record's date: 2023-03-18
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S2212877814002087?via%3Dihub
    Papper original source: Molecular Metabolism. 4 (3): 222-226
    APA: Haeusler, Rebecca A.; Camastra, Stefania; Astiarraga, Brenno; Nannipieri, Monica; Anselmino, Marco; Ferrannini, Ele; (2015). Decreased expression of hepatic glucokinase in type 2 diabetes. Molecular Metabolism, 4(3), 222-226. DOI: 10.1016/j.molmet.2014.12.007
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.molmet.2014.12.007
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2015
    Publication Type: Journal Publications
  • Keywords:

    Cell Biology,Endocrinology & Metabolism,Molecular Biology
    De-novo lipogenesis
    Diabetes
    Fasting hyperglycemia
    Fatty rats
    Foxo1
    Gene-expression
    Glucokinase
    Gluconeogenesis
    Glucose-production
    Humans
    Liver
    Metabolism
    Postprandial hyperglycemia
    Cell biology
    Ciências biológicas ii
    Endocrinology & metabolism
    Molecular biology
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