Articles producció científica> Medicina i Cirurgia

Expression of STING in Women with Morbid Obesity and Nonalcoholic Fatty Liver Disease

  • Dades identificatives

    Identificador: imarina:9296659
    Autors:
    Bertran, LaiaAdalid, LaiaVilaro-Blay, MerceBarrientos-Riosalido, AndreaAguilar, CarmenMartinez, SalomeSabench, Fatimadel Castillo, DanielPorras, Jose AntonioAlibalic, AjlaRichart, CristobalAuguet, Teresa
    Resum:
    Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic hepatic disease. Although mostly benign, this disease can evolve into nonalcoholic steatohepatitis (NASH). The stimulator of interferon genes (STING) plays an important role in the immune response against stressed cells, but this protein may also be involved in liver lipogenesis and microbiota composition. In this study, the role of STING in NAFLD was evaluated by RT–qPCR to analyze STING mRNA abundance and by immunohistochemical analysis to evaluate protein expression in liver biopsies from a cohort composed of 69 women with morbid obesity classified according to their liver involvement (normal liver, n = 27; simple steatosis (SS), n = 26; NASH, n = 16). The results showed that STING mRNA expression in the liver increases with the occurrence of NAFLD, specifically in the SS stage in which the degree of steatosis is mild or moderate. Protein analysis corroborated these results. Positive correlations were observed among hepatic STING mRNA abundance and gamma-glutamyl transferase and alkaline phosphatase levels, hepatic Toll-like receptor 9 expression and some circulating microbiota-derived bile acids. In conclusion, STING may be involved in the outcome and progression of NAFLD and may be related to hepatic lipid metabolism. However, further studies are needed to confirm these findings.
  • Altres:

    Autor segons l'article: Bertran, Laia; Adalid, Laia; Vilaro-Blay, Merce; Barrientos-Riosalido, Andrea; Aguilar, Carmen; Martinez, Salome; Sabench, Fatima; del Castillo, Daniel; Porras, Jose Antonio; Alibalic, Ajla; Richart, Cristobal; Auguet, Teresa
    Departament: Medicina i Cirurgia Ciències Mèdiques Bàsiques
    Autor/s de la URV: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Barrientos Riosalido, Andrea / Bertran Ramos, Laia / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima
    Paraules clau: Stimulator of interferon genes Nonalcoholic fatty liver disease Microbiota Lipogenesis Inflammation stimulator of interferon genes pathway microbiota lipogenesis inflammation cirrhosis
    Resum: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic hepatic disease. Although mostly benign, this disease can evolve into nonalcoholic steatohepatitis (NASH). The stimulator of interferon genes (STING) plays an important role in the immune response against stressed cells, but this protein may also be involved in liver lipogenesis and microbiota composition. In this study, the role of STING in NAFLD was evaluated by RT–qPCR to analyze STING mRNA abundance and by immunohistochemical analysis to evaluate protein expression in liver biopsies from a cohort composed of 69 women with morbid obesity classified according to their liver involvement (normal liver, n = 27; simple steatosis (SS), n = 26; NASH, n = 16). The results showed that STING mRNA expression in the liver increases with the occurrence of NAFLD, specifically in the SS stage in which the degree of steatosis is mild or moderate. Protein analysis corroborated these results. Positive correlations were observed among hepatic STING mRNA abundance and gamma-glutamyl transferase and alkaline phosphatase levels, hepatic Toll-like receptor 9 expression and some circulating microbiota-derived bile acids. In conclusion, STING may be involved in the outcome and progression of NAFLD and may be related to hepatic lipid metabolism. However, further studies are needed to confirm these findings.
    Àrees temàtiques: Molecular biology Medicina ii Farmacia Endocrinology, diabetes and metabolism Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry & molecular biology Biochemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: andrea.barrientos@urv.cat andrea.barrientos@urv.cat joseantonio.porras@urv.cat mariasalome.martinez@urv.cat danieldel.castillo@urv.cat laia.bertranr@estudiants.urv.cat laia.bertranr@estudiants.urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Identificador de l'autor: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-9052-1368 0000-0001-9052-1368 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Data d'alta del registre: 2024-09-28
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/2218-1989/13/4/496
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Metabolites. 13 (4): 496-
    Referència de l'ítem segons les normes APA: Bertran, Laia; Adalid, Laia; Vilaro-Blay, Merce; Barrientos-Riosalido, Andrea; Aguilar, Carmen; Martinez, Salome; Sabench, Fatima; del Castillo, Danie (2023). Expression of STING in Women with Morbid Obesity and Nonalcoholic Fatty Liver Disease. Metabolites, 13(4), 496-. DOI: 10.3390/metabo13040496
    DOI de l'article: 10.3390/metabo13040496
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry,Biochemistry & Molecular Biology,Endocrinology, Diabetes and Metabolism,Molecular Biology
    Stimulator of interferon genes
    Nonalcoholic fatty liver disease
    Microbiota
    Lipogenesis
    Inflammation
    stimulator of interferon genes
    pathway
    microbiota
    lipogenesis
    inflammation
    cirrhosis
    Molecular biology
    Medicina ii
    Farmacia
    Endocrinology, diabetes and metabolism
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
    Biochemistry & molecular biology
    Biochemistry
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