Articles producció científica> Bioquímica i Biotecnologia

The yeast mRNA-binding protein Cth2 post-transcriptionally modulates ergosterol biosynthesis in response to iron deficiency

  • Dades identificatives

    Identificador: imarina:9325567
    Autors:
    Jordá TRozès NMartínez-Pastor MTPuig S
    Resum:
    Sterol synthesis is an iron-dependent metabolic pathway in eukaryotes. Consequently, fungal ergosterol biosynthesis (ERG) is down-regulated in response to iron deficiency. In this report, we show that, upon iron limitation or overexpression of the iron-regulated mRNA-binding protein Cth2, the yeast Saccharomyces cerevisiae down-regulates the three initial enzymatic steps of ergosterol synthesis (ERG1, ERG7 and ERG11). Mechanistically, we show that Cth2 protein limits the translation and promotes the decrease in the mRNA levels of these specific ERG genes, which contain consensus Cth2-binding sites defined as AU-rich elements (AREs). Thus, expression of CTH2 leads to the accumulation of initial sterol intermediates, such as squalene, and to the drop of ergosterol levels. Changes in CTH2 expression levels disturb the response of yeast cells to stresses related to membrane integrity such as high ethanol and sorbitol concentrations. Therefore, CTH2 should be considered as a critical regulatory factor of ergosterol biosynthesis during iron deficiency.Copyright © 2023. Published by Elsevier B.V.
  • Altres:

    Autor segons l'article: Jordá T; Rozès N; Martínez-Pastor MT; Puig S
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: Rozès, Nicolas Andre Louis
    Paraules clau: Yeast Ethanol Ergosterol Erg Cth2 kinase ergosterol erg decay cth2
    Resum: Sterol synthesis is an iron-dependent metabolic pathway in eukaryotes. Consequently, fungal ergosterol biosynthesis (ERG) is down-regulated in response to iron deficiency. In this report, we show that, upon iron limitation or overexpression of the iron-regulated mRNA-binding protein Cth2, the yeast Saccharomyces cerevisiae down-regulates the three initial enzymatic steps of ergosterol synthesis (ERG1, ERG7 and ERG11). Mechanistically, we show that Cth2 protein limits the translation and promotes the decrease in the mRNA levels of these specific ERG genes, which contain consensus Cth2-binding sites defined as AU-rich elements (AREs). Thus, expression of CTH2 leads to the accumulation of initial sterol intermediates, such as squalene, and to the drop of ergosterol levels. Changes in CTH2 expression levels disturb the response of yeast cells to stresses related to membrane integrity such as high ethanol and sorbitol concentrations. Therefore, CTH2 should be considered as a critical regulatory factor of ergosterol biosynthesis during iron deficiency.Copyright © 2023. Published by Elsevier B.V.
    Àrees temàtiques: Structural biology Molecular biology Medicina i Genetics Farmacia Ciências biológicas ii Ciências biológicas i Biotecnología Biophysics Biochemistry & molecular biology Biochemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: nicolasrozes@urv.cat
    Identificador de l'autor: 0000-0001-9718-3429
    Data d'alta del registre: 2024-08-03
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/pii/S1874939923000548
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms. 1866 (3): 194959-194959
    Referència de l'ítem segons les normes APA: Jordá T; Rozès N; Martínez-Pastor MT; Puig S (2023). The yeast mRNA-binding protein Cth2 post-transcriptionally modulates ergosterol biosynthesis in response to iron deficiency. Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 1866(3), 194959-194959. DOI: 10.1016/j.bbagrm.2023.194959
    DOI de l'article: 10.1016/j.bbagrm.2023.194959
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry,Biochemistry & Molecular Biology,Biophysics,Genetics,Molecular Biology,Structural Biology
    Yeast
    Ethanol
    Ergosterol
    Erg
    Cth2
    kinase
    ergosterol
    erg
    decay
    cth2
    Structural biology
    Molecular biology
    Medicina i
    Genetics
    Farmacia
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
    Biophysics
    Biochemistry & molecular biology
    Biochemistry
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