Autor segons l'article: Jordá T; Rozès N; Martínez-Pastor MT; Puig S
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Rozès, Nicolas Andre Louis
Paraules clau: Yeast Ethanol Ergosterol Erg Cth2 kinase ergosterol erg decay cth2
Resum: Sterol synthesis is an iron-dependent metabolic pathway in eukaryotes. Consequently, fungal ergosterol biosynthesis (ERG) is down-regulated in response to iron deficiency. In this report, we show that, upon iron limitation or overexpression of the iron-regulated mRNA-binding protein Cth2, the yeast Saccharomyces cerevisiae down-regulates the three initial enzymatic steps of ergosterol synthesis (ERG1, ERG7 and ERG11). Mechanistically, we show that Cth2 protein limits the translation and promotes the decrease in the mRNA levels of these specific ERG genes, which contain consensus Cth2-binding sites defined as AU-rich elements (AREs). Thus, expression of CTH2 leads to the accumulation of initial sterol intermediates, such as squalene, and to the drop of ergosterol levels. Changes in CTH2 expression levels disturb the response of yeast cells to stresses related to membrane integrity such as high ethanol and sorbitol concentrations. Therefore, CTH2 should be considered as a critical regulatory factor of ergosterol biosynthesis during iron deficiency.Copyright © 2023. Published by Elsevier B.V.
Àrees temàtiques: Structural biology Molecular biology Medicina i Genetics Farmacia Ciências biológicas ii Ciências biológicas i Biotecnología Biophysics Biochemistry & molecular biology Biochemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: nicolasrozes@urv.cat
Identificador de l'autor: 0000-0001-9718-3429
Data d'alta del registre: 2024-08-03
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/pii/S1874939923000548
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms. 1866 (3): 194959-194959
Referència de l'ítem segons les normes APA: Jordá T; Rozès N; Martínez-Pastor MT; Puig S (2023). The yeast mRNA-binding protein Cth2 post-transcriptionally modulates ergosterol biosynthesis in response to iron deficiency. Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 1866(3), 194959-194959. DOI: 10.1016/j.bbagrm.2023.194959
DOI de l'article: 10.1016/j.bbagrm.2023.194959
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2023
Tipus de publicació: Journal Publications