Autor segons l'article: Ortiz, Mayreli; Jauset-Rubio, Miriam; Trummer, Olivia; Foessl, Ines; Kodr, David; Acero, Josep Lluis; Botero, Mary Luz; Biggs, Phil; Lenartowicz, Daniel; Trajanoska, Katerina; Rivadeneira, Fernando; Hocek, Michal; Obermayer-Pietsch, Barbara; O'Sullivan, Ciara K
Departament: Enginyeria Química
Autor/s de la URV: ACERO SÁNCHEZ, JOSEP LLUÍS / BOTERO GALLEGO, MARY LUZ / Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli
Paraules clau: Risk Markers Identification Fracture Extension Epidemiology Dna Chain-reaction Bone-mineral density Amplification
Resum: Generic platformusing isothermal solid-phase primer elongationfor cost-effective, rapid electrochemical SNP genotyping from a fingerprickblood sample.Osteoporosis is amultifactorial disease influenced by geneticand environmental factors, which contributes to an increased riskof bone fracture, but early diagnosis of this disease cannot be achievedusing current techniques. We describe a generic platform for the targetedelectrochemical genotyping of SNPs identified by genome-wide associationstudies to be associated with a genetic predisposition to osteoporosis.The platform exploits isothermal solid-phase primer elongation withferrocene-labeled nucleoside triphosphates. Thiolated reverse primersdesigned for each SNP were immobilized on individual gold electrodesof an array. These primers are designed to hybridize to the SNP siteat their 3 & PRIME;OH terminal, and primer elongation occurs only wherethere is 100% complementarity, facilitating the identification andheterozygosity of each SNP under interrogation. The platform was appliedto real blood samples, which were thermally lysed and directly usedwithout the need for DNA extraction or purification. The results werevalidated using Taqman SNP genotyping assays and Sanger sequencing.The assay is complete in just 15 min with a total cost of 0.3europer electrode. The platform is completely generic and has immensepotential for deployment at the point of need in an automated devicefor targeted SNP genotyping with the only required end-user interventionbeing sample addition.
Àrees temàtiques: General chemistry General chemical engineering Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemical engineering (miscellaneous) Chemical engineering (all)
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: mayreli.ortiz@urv.cat miriam.jauset@urv.cat mayreli.ortiz@urv.cat
Identificador de l'autor: 0000-0002-9423-0055 0000-0002-9943-6132 0000-0002-9423-0055
Data d'alta del registre: 2024-10-26
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://pubs.acs.org/doi/10.1021/acscentsci.3c00243
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Acs Central Science. 9 (8): 1591-1602
Referència de l'ítem segons les normes APA: Ortiz, Mayreli; Jauset-Rubio, Miriam; Trummer, Olivia; Foessl, Ines; Kodr, David; Acero, Josep Lluis; Botero, Mary Luz; Biggs, Phil; Lenartowicz, Dani (2023). Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates. Acs Central Science, 9(8), 1591-1602. DOI: 10.1021/acscentsci.3c00243
DOI de l'article: 10.1021/acscentsci.3c00243
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2023
Tipus de publicació: Journal Publications