Articles producció científica> Enginyeria Química

Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition

  • Dades identificatives

    Identificador: imarina:9329034
    Autors:
    Kodr, DavidOrtiz, MayreliSykorova, VeronikaYenice, Cansu PinarLesnikowski, Zbigniew JO'Sullivan, Ciara KHocek, Michal
    Resum:
    The previously reported approach of orthogonal multipotentialredoxcoding of all four DNA bases allowed only analysis of the relativenucleotide composition of short DNA stretches. Here, we present twomethods for normalization of the electrochemical readout to facilitatethe determination of the total nucleotide composition. The first methodis based on the presence or absence of an internal standard of 7-deaza-2 & PRIME;-deoxyguanosinein a DNA primer. The exact composition of the DNA was elucidated upontwo parallel analyses and the subtraction of the electrochemical signalintensities. The second approach took advantage of a 5 & PRIME;-viologenmodified primer, with this fifth orthogonal redox label acting asa reference for signal normalization, thus allowing accurate electrochemicalsequence analysis in a single read. Both approaches were tested usingvarious sequences, and the voltammetric signals obtained were normalizedusing either the internal standard or the reference label and demonstratedto be in perfect agreement with the actual nucleotide composition,highlighting the potential for targeted DNA sequence analysis.
  • Altres:

    Autor segons l'article: Kodr, David; Ortiz, Mayreli; Sykorova, Veronika; Yenice, Cansu Pinar; Lesnikowski, Zbigniew J; O'Sullivan, Ciara K; Hocek, Michal
    Departament: Enginyeria Química
    Autor/s de la URV: O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli / Yenice, Cansu Pinar
    Paraules clau: Tyrosine Sensors Nucleoside triphosphates Direct electrochemical detection Aromatic-groups
    Resum: The previously reported approach of orthogonal multipotentialredoxcoding of all four DNA bases allowed only analysis of the relativenucleotide composition of short DNA stretches. Here, we present twomethods for normalization of the electrochemical readout to facilitatethe determination of the total nucleotide composition. The first methodis based on the presence or absence of an internal standard of 7-deaza-2 & PRIME;-deoxyguanosinein a DNA primer. The exact composition of the DNA was elucidated upontwo parallel analyses and the subtraction of the electrochemical signalintensities. The second approach took advantage of a 5 & PRIME;-viologenmodified primer, with this fifth orthogonal redox label acting asa reference for signal normalization, thus allowing accurate electrochemicalsequence analysis in a single read. Both approaches were tested usingvarious sequences, and the voltammetric signals obtained were normalizedusing either the internal standard or the reference label and demonstratedto be in perfect agreement with the actual nucleotide composition,highlighting the potential for targeted DNA sequence analysis.
    Àrees temàtiques: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: cansupinar.yenice@urv.cat mayreli.ortiz@urv.cat mayreli.ortiz@urv.cat cansupinar.yenice@urv.cat
    Identificador de l'autor: 0000-0002-9423-0055 0000-0002-9423-0055
    Data d'alta del registre: 2024-10-26
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://pubs.acs.org/doi/10.1021/acs.analchem.3c02023
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Analytical Chemistry. 95 (34): 12586-12589
    Referència de l'ítem segons les normes APA: Kodr, David; Ortiz, Mayreli; Sykorova, Veronika; Yenice, Cansu Pinar; Lesnikowski, Zbigniew J; O'Sullivan, Ciara K; Hocek, Michal (2023). Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition. Analytical Chemistry, 95(34), 12586-12589. DOI: 10.1021/acs.analchem.3c02023
    DOI de l'article: 10.1021/acs.analchem.3c02023
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Analytical Chemistry,Chemistry, Analytical
    Tyrosine
    Sensors
    Nucleoside triphosphates
    Direct electrochemical detection
    Aromatic-groups
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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