Repositori URV

Identificador: imarina:9332029

Handle: https://hdl.handle.net/20.500.11797/imarina9332029

Autors:
Kovatcheva, MartaMelendez, ElenaChondronasiou, DafniPietrocola, FedericoBernad, RaquelCaballe, AdriaJunza, AlexandraCapellades, JordiHolguin-Horcajo, AdrianPrats, NeusDurand, SylvereRovira, MeritxellYanes, OscarStephan-Otto Attolini, CamilleKroemer, GuidoSerrano, Manuel

Resum:
Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its metabolic requirements. Here we show that OSKM reprogramming in mice causes a global depletion of vitamin B12 and molecular hallmarks of methionine starvation. Supplementation with vitamin B12 increases the efficiency of reprogramming both in mice and in cultured cells, the latter indicating a cell-intrinsic effect. We show that the epigenetic mark H3K36me3, which prevents illegitimate initiation of transcription outside promoters (cryptic transcription), is sensitive to vitamin B12 levels, providing evidence for a link between B12 levels, H3K36 methylation, transcriptional fidelity and efficient reprogramming. Vitamin B12 supplementation also accelerates tissue repair in a model of ulcerative colitis. We conclude that vitamin B12, through its key role in one-carbon metabolism and epigenetic dynamics, improves the efficiency of in vivo reprogramming and tissue repair.© 2023. The Author(s).