Autor según el artículo: Kovatcheva, Marta; Melendez, Elena; Chondronasiou, Dafni; Pietrocola, Federico; Bernad, Raquel; Caballe, Adria; Junza, Alexandra; Capellades, Jordi; Holguin-Horcajo, Adrian; Prats, Neus; Durand, Sylvere; Rovira, Meritxell; Yanes, Oscar; Stephan-Otto Attolini, Camille; Kroemer, Guido; Serrano, Manuel
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Junza Martínez, Alexandra / Yanes Torrado, Óscar
Palabras clave: Wound healing Vitamins Vitamin b 12 Stem-cells Mice Cellular reprogramming Cell plasticity Animals regeneration methylation metabolism longevity identification gut deficiency cancer alignment
Resumen: Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its metabolic requirements. Here we show that OSKM reprogramming in mice causes a global depletion of vitamin B12 and molecular hallmarks of methionine starvation. Supplementation with vitamin B12 increases the efficiency of reprogramming both in mice and in cultured cells, the latter indicating a cell-intrinsic effect. We show that the epigenetic mark H3K36me3, which prevents illegitimate initiation of transcription outside promoters (cryptic transcription), is sensitive to vitamin B12 levels, providing evidence for a link between B12 levels, H3K36 methylation, transcriptional fidelity and efficient reprogramming. Vitamin B12 supplementation also accelerates tissue repair in a model of ulcerative colitis. We conclude that vitamin B12, through its key role in one-carbon metabolism and epigenetic dynamics, improves the efficiency of in vivo reprogramming and tissue repair.© 2023. The Author(s).
Áreas temáticas: Physiology (medical) Internal medicine Endocrinology, diabetes and metabolism Endocrinology & metabolism Cell biology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: oscar.yanes@urv.cat alexandra.junza@urv.cat
Identificador del autor: 0000-0003-3695-7157 0000-0001-7205-0419
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.nature.com/articles/s42255-023-00916-6
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Nature Metabolism. 5 (11): 1911-1930
Referencia de l'ítem segons les normes APA: Kovatcheva, Marta; Melendez, Elena; Chondronasiou, Dafni; Pietrocola, Federico; Bernad, Raquel; Caballe, Adria; Junza, Alexandra; Capellades, Jordi; H (2023). Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair. Nature Metabolism, 5(11), 1911-1930. DOI: 10.1038/s42255-023-00916-6
DOI del artículo: 10.1038/s42255-023-00916-6
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications