Autor segons l'article: Moreno-Vedia, J; Llop, D; Rodríguez-Calvo, R; Plana, N; Amigó, N; Rosales, R; Esteban, Y; Masana, L; Ibarretxe, D; Girona, J
Departament: Ciències Mèdiques Bàsiques Medicina i Cirurgia
Autor/s de la URV: Girona Tell, Josefa / Ibarretxe Gerediaga, Daiana / Llop Paredes, Dídac / Masana Marín, Luis / Moreno Vedia, Juan / Plana Gil, Núria / Rodríguez Calvo, Ricardo / ROSALES RIBAS, ROSER
Paraules clau: Triglyceride-rich lipoproteins Macrophages Inflammation H-1-nmr Atherosclerotic cardiovascular-disease Atherosclerosis 1h-nmr 1 h-nmr triglyceride-rich lipoproteins risk macrophages low-grade inflammation insights inflammation cholesterol atherosclerosis
Resum: Triglyceride-rich lipoproteins (TRLs) can have an important role in atherosclerosis development due to their size and ability to penetrate the endothelium. While high plasma triglyceride (TG) levels and chronic inflammation are relevant in metabolic diseases, it remains unclear whether TGs are atherogenic or which TRL-TG-derived metabolites are responsible for inflammation. Here, we aimed to study the lipidome modifications of TRL particles enriched in TG in patients with hyperlipidemia and their associations with a proinflammatory status both in vivo and in vitro.Using proton nuclear magnetic resonance (1 H-NMR), we analysed the plasma levels of glycoprotein acetyls and the TRL lipidomic profile of 307 patients with dyslipidemia. THP-1-derived macrophages were used as an in vitro model to explore the molecular inflammatory effects mediated by TRL.In vivo, higher TRL-TG levels were associated with higher circulating levels of NMR-measured glycoproteins (Glyc-A, Glyc-B and Glyc-F; p < .001). Lipidomic analysis showed that TRL-TG enrichment led to decreased cholesterol and phospholipid content (p < .01), an increase in omega-9, and a decrease in saturated fatty acids (p < .001). THP-1 macrophages exposed to increasing TRL particle concentrations augmented the secretion of IL-1β and TNF-α, which varied based on particle composition. Particles with higher cholesterol and phospholipid contents exerted higher cytokine secretion. The activation of MAPK, Akt/NFκB, and caspase-1 was concurrent with this proinflammatory response.High TRL-TG levels are associated with a higher systemic inflammatory status and increased particle concentrations. In vitro, higher particle numbers increase proinflammatory cytokine secretion, with cholesterol and phospholipid-rich TRL being more proinflammatory.© 2023 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.
Àrees temàtiques: Saúde coletiva Odontología Nutrição Medicine, research & experimental Medicine, general & internal Medicine (miscellaneous) Medicina ii Medicina i Interdisciplinar General medicine Farmacia Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: daiana.ibarretxe@urv.cat luis.masana@urv.cat didac.llop@urv.cat josefa.girona@urv.cat ricardo.rodriguez@urv.cat didac.llop@urv.cat josefa.girona@urv.cat juan.moreno@estudiants.urv.cat juan.moreno@estudiants.urv.cat
Identificador de l'autor: 0000-0002-0789-4954 0000-0002-6267-8779 0000-0002-6267-8779 0000-0002-2673-3822 0000-0002-2673-3822
Data d'alta del registre: 2024-08-03
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: European Journal Of Clinical Investigation. 54 (3): e14132-e14132
Referència de l'ítem segons les normes APA: Moreno-Vedia, J; Llop, D; Rodríguez-Calvo, R; Plana, N; Amigó, N; Rosales, R; Esteban, Y; Masana, L; Ibarretxe, D; Girona, J (2024). Lipidomics of triglyceride-rich lipoproteins derived from hyperlipidemic patients on inflammation. European Journal Of Clinical Investigation, 54(3), e14132-e14132. DOI: 10.1111/eci.14132
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2024
Tipus de publicació: Journal Publications
Repositori URV