Articles producció científica> Bioquímica i Biotecnologia

Exploring Metabolic and Gut Microbiome Responses to Paraquat Administration in Male Wistar Rats: Implications for Oxidative Stress

  • Dades identificatives

    Identificador: imarina:9351849
    Autors:
    Hernandez-Baixauli, JChomiciute, GTracey, HMora, ICortés-Espinar, AJAvila-Román, JAbasolo, NPalacios-Jordan, HFoguet-Romero, ESuñol, DGalofré, MAlcaide-Hidalgo, JMBaselga-Escudero, Ldel Bas, JMMulero, M
    Resum:
    In this study, we examined the metabolic and gut microbiome responses to paraquat (PQ) in male Wistar rats, focusing on oxidative stress effects. Rats received a single intraperitoneal injection of PQ at 15 and 30 mg/kg, and various oxidative stress parameters (i.e., MDA, SOD, ROS, 8-isoprostanes) were assessed after three days. To explore the omic profile, GC-qTOF and UHPLC-qTOF were performed to assess the plasma metabolome; 1H-NMR was used to assess the urine metabolome; and shotgun metagenomics sequencing was performed to study the gut microbiome. Our results revealed reductions in body weight and tissue changes, particularly in the liver, were observed, suggesting a systemic effect of PQ. Elevated lipid peroxidation and reactive oxygen species levels in the liver and plasma indicated the induction of oxidative stress. Metabolic profiling revealed changes in the tricarboxylic acid cycle, accumulation of ketone body, and altered levels of key metabolites, such as 3-hydroxybutyric acid and serine, suggesting intricate links between energy metabolism and redox reactions. Plasma metabolomic analysis revealed alterations in mitochondrial metabolism, nicotinamide metabolism, and tryptophan degradation. The gut microbiome showed shifts, with higher PQ doses influencing microbial populations (e.g., Escherichia coli and Akkermansia muciniphila) and metagenomic functions (pyruvate metabolism, fermentation, nucleotide and amino acid biosynthesis). Overall, this study provides comprehensive insights into the complex interplay between PQ exposure, metabolic responses, and gut microbiome dynamics. These findings enhance our understanding of the mechanisms behind oxidative stress-induced metabolic alterations and underscore the connections between xenobiotic exposure, gut microbio
  • Altres:

    Autor segons l'article: Hernandez-Baixauli, J; Chomiciute, G; Tracey, H; Mora, I; Cortés-Espinar, AJ; Avila-Román, J; Abasolo, N; Palacios-Jordan, H; Foguet-Romero, E; Suñol, D; Galofré, M; Alcaide-Hidalgo, JM; Baselga-Escudero, L; del Bas, JM; Mulero, M
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: Ávila Román, Francisco Javier / BASELGA ESCUDERO, LAURA
    Paraules clau: Sphingomyelins Paraquat Oxidative stress Metabolism Lysophospholipids Gut microbiome Escherichia coli Akkermansia-muciniphila Akkermansia muciniphila 3-hydroxibutiric acid toxicity sphingomyelins peroxide oxidative stress metabolism lysophospholipids ketone-bodies inhibition health gut microbiome features exposure escherichia coli enzymes children akkermansia muciniphila 3-hydroxibutiric acid
    Resum: In this study, we examined the metabolic and gut microbiome responses to paraquat (PQ) in male Wistar rats, focusing on oxidative stress effects. Rats received a single intraperitoneal injection of PQ at 15 and 30 mg/kg, and various oxidative stress parameters (i.e., MDA, SOD, ROS, 8-isoprostanes) were assessed after three days. To explore the omic profile, GC-qTOF and UHPLC-qTOF were performed to assess the plasma metabolome; 1H-NMR was used to assess the urine metabolome; and shotgun metagenomics sequencing was performed to study the gut microbiome. Our results revealed reductions in body weight and tissue changes, particularly in the liver, were observed, suggesting a systemic effect of PQ. Elevated lipid peroxidation and reactive oxygen species levels in the liver and plasma indicated the induction of oxidative stress. Metabolic profiling revealed changes in the tricarboxylic acid cycle, accumulation of ketone body, and altered levels of key metabolites, such as 3-hydroxybutyric acid and serine, suggesting intricate links between energy metabolism and redox reactions. Plasma metabolomic analysis revealed alterations in mitochondrial metabolism, nicotinamide metabolism, and tryptophan degradation. The gut microbiome showed shifts, with higher PQ doses influencing microbial populations (e.g., Escherichia coli and Akkermansia muciniphila) and metagenomic functions (pyruvate metabolism, fermentation, nucleotide and amino acid biosynthesis). Overall, this study provides comprehensive insights into the complex interplay between PQ exposure, metabolic responses, and gut microbiome dynamics. These findings enhance our understanding of the mechanisms behind oxidative stress-induced metabolic alterations and underscore the connections between xenobiotic exposure, gut microbiota, and host metabolism.
    Àrees temàtiques: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: franciscojavier.avila@urv.cat
    Identificador de l'autor: 0000-0001-9766-8178
    Data d'alta del registre: 2024-02-17
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/2076-3921/13/1/67
    Referència a l'article segons font original: Antioxidants. 13 (1):
    Referència de l'ítem segons les normes APA: Hernandez-Baixauli, J; Chomiciute, G; Tracey, H; Mora, I; Cortés-Espinar, AJ; Avila-Román, J; Abasolo, N; Palacios-Jordan, H; Foguet-Romero, E; Suñol, (2024). Exploring Metabolic and Gut Microbiome Responses to Paraquat Administration in Male Wistar Rats: Implications for Oxidative Stress. Antioxidants, 13(1), -. DOI: 10.3390/antiox13010067
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.3390/antiox13010067
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2024
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry,Biochemistry & Molecular Biology,Cell Biology,Chemistry, Medicinal,Clinical Biochemistry,Food Science,Food Science & Technology,Molecular Biology,Physiology
    Sphingomyelins
    Paraquat
    Oxidative stress
    Metabolism
    Lysophospholipids
    Gut microbiome
    Escherichia coli
    Akkermansia-muciniphila
    Akkermansia muciniphila
    3-hydroxibutiric acid
    toxicity
    sphingomyelins
    peroxide
    oxidative stress
    metabolism
    lysophospholipids
    ketone-bodies
    inhibition
    health
    gut microbiome
    features
    exposure
    escherichia coli
    enzymes
    children
    akkermansia muciniphila
    3-hydroxibutiric acid
    Química
    Physiology
    Molecular biology
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Clinical biochemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, medicinal
    Cell biology
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Biochemistry
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