Author, as appears in the article.: M.J. Figueras; F. Latif-Eugenín; R. Beaz-Hidalgo
Department: Ciències Mèdiques Bàsiques
URV's Author/s: FIGUERAS SALVAT, MARIA JOSEFA; F. Latif-Eugenín; BEAZ HIDALGO, ROXANA
Keywords: Aeromonas shellfish Water
Abstract: Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. Methods and Results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. Significance and Impact of the Study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.
Research group: Unitat de Micologia i Microbiologia Ambiental
Thematic Areas: Ciències de la salut Ciencias de la salud Health sciences
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1364-5072
Author identifier: N/D; N/D; N/D
Record's date: 2016-09-21
Last page: 891
Journal volume: 121
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.13210
Article's DOI: 10.1111/jam.13210
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
First page: 883
Publication Type: Article Artículo Article
Repositori URV