Author, as appears in the article.: Suárez-García, S.; Arola, L.; Pascual-Serrano, A.; Arola-Arnal, A.; Aragonès, G.; Bladé, C.; Suárez, M.
Department: Bioquímica i Biotecnologia
URV's Author/s: SUÁREZ GARCÍA, SUSANA; AROLA FERRER, LUIS MARIA; PASCUAL SERRANO, AÏDA; AROLA ARNAL, ANNA; ARAGONÈS BARGALLÓ, GERARD; BLADÉ SEGARRA, MARIA CINTA; SUÁREZ RECIO, MANUEL
Keywords: Biomarkers Cafeteria diet Glycerophospholipid
Abstract: Recent investigations based on non-targeted metabolomics have proposed lysophospholipids (Lyso-PLs) as biomarkers of different diseases. In particular, lysophosphatidylcholines (Lyso-PCs) and lysophosphatidylethanolamines (Lyso-PEs) have been associated with serious lipid pathologies. Methods to determine the different molecular species in a biological sample and to quantify even less abundant species are required for the evaluation of the Lyso-PL pattern as a novel comprehensive biomarker of dyslipidemia. This study describes the development and validation of an ultra-high-performance liquid chromatography coupled to tandem mass spectrometry assay for the determination of a large number of Lyso-PCs and Lyso-PEs in biological samples. The method was validated in rat serum using two simple methanol-based extractions with low sample volumes (5–50 μL) that covered the wide concentration range of these metabolites. In total, thirty-one Lyso-PLs were separated and quantified with low method limits of detection and quantification, reaching values of 0.2 and 0.8 nM, respectively. The method was subsequently applied in the identification of Lyso-PL-related changes produced by the chronic intake of a cafeteria diet. The results showed alterations in the majority of Lyso-PCs and Lyso-PEs in rat serum. Furthermore, multivariate analysis indicated that the comprehensive evaluation of serum Lyso-PLs could be an excellent indicator of the nutritional phenotype associated with an increased risk of lipid disorders.
Research group: Grup de Recerca en Nutrigenòmica Unitat de Recerca Biomèdica
Thematic Areas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1570-0232
Author identifier: ; 0000-0003-2767-1974; ; 0000-0001-6529-1345; 0000-0001-8657-5726; 0000-0003-2838-2402;
Record's date: 2017-05-17
Last page: 97
Journal volume: 1055-1056
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: http://www.sciencedirect.com/science/article/pii/S1570023217303082
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1016/j.jchromb.2017.04.028
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
First page: 86
Publication Type: Article Artículo Article
Repositori URV