Autor según el artículo: Suárez-García, S.; Arola, L.; Pascual-Serrano, A.; Arola-Arnal, A.; Aragonès, G.; Bladé, C.; Suárez, M.

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: SUÁREZ GARCÍA, SUSANA; AROLA FERRER, LUIS MARIA; PASCUAL SERRANO, AÏDA; AROLA ARNAL, ANNA; ARAGONÈS BARGALLÓ, GERARD; BLADÉ SEGARRA, MARIA CINTA; SUÁREZ RECIO, MANUEL

Palabras clave: Biomarkers Cafeteria diet Glycerophospholipid

Resumen: Recent investigations based on non-targeted metabolomics have proposed lysophospholipids (Lyso-PLs) as biomarkers of different diseases. In particular, lysophosphatidylcholines (Lyso-PCs) and lysophosphatidylethanolamines (Lyso-PEs) have been associated with serious lipid pathologies. Methods to determine the different molecular species in a biological sample and to quantify even less abundant species are required for the evaluation of the Lyso-PL pattern as a novel comprehensive biomarker of dyslipidemia. This study describes the development and validation of an ultra-high-performance liquid chromatography coupled to tandem mass spectrometry assay for the determination of a large number of Lyso-PCs and Lyso-PEs in biological samples. The method was validated in rat serum using two simple methanol-based extractions with low sample volumes (5–50 μL) that covered the wide concentration range of these metabolites. In total, thirty-one Lyso-PLs were separated and quantified with low method limits of detection and quantification, reaching values of 0.2 and 0.8 nM, respectively. The method was subsequently applied in the identification of Lyso-PL-related changes produced by the chronic intake of a cafeteria diet. The results showed alterations in the majority of Lyso-PCs and Lyso-PEs in rat serum. Furthermore, multivariate analysis indicated that the comprehensive evaluation of serum Lyso-PLs could be an excellent indicator of the nutritional phenotype associated with an increased risk of lipid disorders.

Grupo de investigación: Grup de Recerca en Nutrigenòmica Unitat de Recerca Biomèdica

Áreas temáticas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1570-0232

Identificador del autor: ; 0000-0003-2767-1974; ; 0000-0001-6529-1345; 0000-0001-8657-5726; 0000-0003-2838-2402;

Fecha de alta del registro: 2017-05-17

Página final: 97

Volumen de revista: 1055-1056

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: http://www.sciencedirect.com/science/article/pii/S1570023217303082

DOI del artículo: 10.1016/j.jchromb.2017.04.028

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Página inicial: 86

Tipo de publicación: Article Artículo Article