Articles producció científica> Química Analítica i Química Orgànica

Artificial receptors for the electrochemical detection of bacterial flagellar filaments from Proteus mirabilis

  • Identification data

    Identifier: PC:3239
    Authors:
    Rius, F. XavierKhan, M. Azizur R.Aires Cardoso, Ana RitaSales, M. Goreti F.Merino, SusanaTomás, Juan M.Riu, J
    Abstract:
    In this paper for the first time we successfully detect bacterial flagellar filaments from Proteus mirabilis using molecularly imprinted artificial receptors. These receptors acted as a sensing layer of the biosensors, assembled by imprinting flagellar proteins onto a polymeric backbone of electropolymerized phenol. In short, flagellar filaments were absorbed onto a carbon support, phenol was electropolymerized around it through the carbon conductive matrix to create the protein molecular molds, and finally the flagellar proteins were removed by enzymatic and electrochemical action. Each removed flagellar protein gave rise to an imprinted site with eventual rebinding ability. Electrical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed to measure the interaction of flagellar filaments with the sensing layer assembled on commercial screen-printed electrodes, providing low detection limits, high precision and selectivity toward the targeted protein. The detection limit was 0.7 ng/mL by EIS and 0.9 ng/mL by SWV. The artificial receptors were further assembled on home-made paper-printed electrodes, with the three-electrode system printed on a paper substrate, offering the possibility of detecting flagellar filaments at as low as 0.6 ng/mL with a disposable and cost-effective portable device. To the best of our knowledge this is the first sensing device where molecularly imprinted artificial receptors are tailored on home-made electrode based on paper substrates with three electrodes assembled together, which is a suitable approach for the fabrication of easy and cost-effective tailored electrodes.
  • Others:

    Author, as appears in the article.: Rius, F. Xavier; Khan, M. Azizur R. ; Aires Cardoso, Ana Rita; Sales, M. Goreti F.; Merino, Susana; Tomás, Juan M. ; Riu, J
    Department: Química Analítica i Química Orgànica
    URV's Author/s: RIUS FERRÚS, FRANCISCO JAVIER; Khan, M. Azizur R. ; Aires Cardoso, Ana Rita; Sales, M. Goreti F.; Merino, Susana; Tomás, Juan M. ; RIU RUSELL, JORDI
    Abstract: In this paper for the first time we successfully detect bacterial flagellar filaments from Proteus mirabilis using molecularly imprinted artificial receptors. These receptors acted as a sensing layer of the biosensors, assembled by imprinting flagellar proteins onto a polymeric backbone of electropolymerized phenol. In short, flagellar filaments were absorbed onto a carbon support, phenol was electropolymerized around it through the carbon conductive matrix to create the protein molecular molds, and finally the flagellar proteins were removed by enzymatic and electrochemical action. Each removed flagellar protein gave rise to an imprinted site with eventual rebinding ability. Electrical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed to measure the interaction of flagellar filaments with the sensing layer assembled on commercial screen-printed electrodes, providing low detection limits, high precision and selectivity toward the targeted protein. The detection limit was 0.7 ng/mL by EIS and 0.9 ng/mL by SWV. The artificial receptors were further assembled on home-made paper-printed electrodes, with the three-electrode system printed on a paper substrate, offering the possibility of detecting flagellar filaments at as low as 0.6 ng/mL with a disposable and cost-effective portable device. To the best of our knowledge this is the first sensing device where molecularly imprinted artificial receptors are tailored on home-made electrode based on paper substrates with three electrodes assembled together, which is a suitable approach for the fabrication of easy and cost-effective tailored electrodes.
    Research group: Grup de Quimiometria, Qualimetria i Nanosensors
    Thematic Areas: Química Química Chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0925-4005
    Author identifier: 0000-0003-4830-1366; ; ; ; ; ; 0000-0001-5823-9223
    Record's date: 2018-04-26
    Last page: 741
    Journal volume: 244
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0925400517300187?via%3Dihub
    Funding program: plan; CTQ2010-18717 altres; PhD Fellowship; Gencat FI; 2013FI_B00842
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.snb.2017.01.018
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    First page: 732
    Publication Type: Article Artículo Article
  • Keywords:

    Sensors químics
    Química
    Química
    Chemistry
    0925-4005
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