Pressurised liquid extraction and liquid chromatography-high resolution mass spectrometry to determine high-intense sweeteners in fish samples

Identifier:  PC:3400

Handle: https://hdl.handle.net/20.500.11797/PC3400

Authors:  Fontanals, N.; Núñez, M.; Borrull, F.; Pocurull, E.

Abstract:
An analytical method based on pressurized liquid extraction (PLE) followed by liquid chromatography-high resolution mass spectrometry (Orbitrap) was developed for the simultaneous determination of ten high-intensity sweeteners in fish samples. As the method was developed, the different PLE parameters were optimized and different clean-up strategies were evaluated, of which in-cell clean-up using alumina and on-cell clean-up with hexane were the most effective. PLE recoveries were between 43% and 94%. The limits of quantification were between 12.5 ng g-1 dry weight (d.w.) and 250 ng g-1 (d.w.) and the limits of detection between 2.5 ng g-1 (d.w.) and 125 ng g-1 (d.w.). Intra-day precision and inter-day precision were below 16% and 25%, respectively. Fish samples from different species were analysed and, saccharin was found below its limit of quantification in the species Scomber scombrus.