Author, as appears in the article.: Espinel-Ingroff A; Pfaller M; Bustamante B; Canton E; Fothergill A; Fuller J; Gonzalez G; Lass-Flörl C; Lockhart S; Martin-Mazuelos E; Meis J; Melhem M; Ostrosky-Zeichner L; Pelaez T; Szeszs M; St-Germain G; Bonfietti L; Guarro J; Turnidge J
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Guarro Artigas, Josep
Keywords: Wild-type Voriconazole Susceptibility Mic distributions Mechanisms Itraconazole In-vitro Broth microdilution method Azole resistance Antifungal agents Amphotericin-b
Abstract: Although epidemiological cutoff values (ECVs) have been established for Candida spp. and the triazoles, they are based on MIC data from a single laboratory. We have established ECVs for eight Candida species and fluconazole, posaconazole, and voriconazole based on wild-type (WT) MIC distributions for isolates of C. albicans (n = 11,241 isolates), C. glabrata (7,538), C. parapsilosis (6,023), C. tropicalis (3,748), C. krusei (1,073), C. lusitaniae (574), C. guilliermondii (373), and C. dubliniensis (162). The 24-h CLSI broth microdilution MICs were collated from multiple laboratories (in Canada, Brazil, Europe, Mexico, Peru, and the United States). The ECVs for distributions originating from ≥6 laboratories, which included ≥95% of the modeled WT population, for fluconazole, posaconazole, and voriconazole were, respectively, 0.5, 0.06 and 0.03 μg/ml for C. albicans, 0.5, 0.25, and 0.03 μg/ml for C. dubliniensis, 8, 1, and 0.25 μg/ml for C. glabrata, 8, 0.5, and 0.12 μg/ml for C. guilliermondii, 32, 0.5, and 0.25 μg/ml for C. krusei, 1, 0.06, and 0.06 μg/ml for C. lusitaniae, 1, 0.25, and 0.03 μg/ml for C. parapsilosis, and 1, 0.12, and 0.06 μg/ml for C. tropicalis. The low number of MICs (<100) for other less prevalent species (C. famata, C. kefyr, C. orthopsilosis, C. rugosa) precluded ECV definition, but their MIC distributions are documented. Evaluation of our ECVs for some species/agent combinations using published individual MICs for 136 isolates (harboring mutations in or upregulation of ERG11, MDR1, CDR1, or CDR2) and 64 WT isolates indicated that our ECVs may be useful in distinguishing WT from non-WT isolates.
Thematic Areas: Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Pharmacology Odontología Nutrição Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Infectious diseases Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: josep.guarro@urv.cat
Author identifier: 0000-0002-7839-7568
Record's date: 2024-07-20
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://journals.asm.org/doi/10.1128/aac.02615-13
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Antimicrobial Agents And Chemotherapy. 58 (4): 2006-2012
APA: Espinel-Ingroff A; Pfaller M; Bustamante B; Canton E; Fothergill A; Fuller J; Gonzalez G; Lass-Flörl C; Lockhart S; Martin-Mazuelos E; Meis J; Melhem (2014). Multilaboratory study of epidemiological cutoff values for detection of resistance in eight Candida species to fluconazole, posaconazole, and voriconazole. Antimicrobial Agents And Chemotherapy, 58(4), 2006-2012. DOI: 10.1128/AAC.02615-13
Article's DOI: 10.1128/AAC.02615-13
Entity: Universitat Rovira i Virgili
Journal publication year: 2014
Publication Type: Journal Publications