Author, as appears in the article.: Martinez-Micaelo, Neus; Gonzalez-Abuin, Noemi; Terra, Ximena; Ardevol, Ana; Pinent, Montserrat; Petretto, Enrico; Behmoaras, Jacques; Blay, Mayte
Department: Bioquímica i Biotecnologia
URV's Author/s: Ardévol Grau, Anna / Blay Olivé, Maria Teresa / Martínez Micaelo, Nieves Beatriz / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Keywords: Wky Monocyte transcriptome Lew Inbred rats Cafeteria diet monocyte transcriptome lew inbred rats cafeteria diet
Abstract: Obesity has reached pandemic levels worldwide. The current models of diet-induced obesity in rodents use predominantly high-fat based diets that do not take into account the consumption of variety of highly palatable, energy-dense foods that are prevalent in Western society. We and others have shown that the cafeteria (CAF) diet is a robust and reproducible model of human metabolic syndrome with tissue inflammation in the rat. We have previously shown that inbred rat strains such as Wistar Kyoto (WKY) and Lewis (LEW) show different susceptibilities to CAF diets with distinct metabolic and morphometric profiles. Here, we show a difference in plasma MCP-1 levels and investigate the effect of the CAF diet on peripheral blood monocyte transcriptome, as powerful stress-sensing immune cells, in WKY and LEW rats. We found that 75.5% of the differentially expressed transcripts under the CAF diet were upregulated in WKY rats and were functionally related to the activation of the immune response. Using a gene co-expression network constructed from the genes differentially expressed between CAF diet-fed LEW and WKY rats, we identified acyl-CoA synthetase short-chain family member 2 (Acss2) as a hub gene for a nutrient-sensing cluster of transcripts in monocytes. The Acss2 genomic region is significantly enriched for previously established metabolism quantitative trait loci in the rat. Notably, monocyte expression levels of Acss2 significantly correlated with plasma glucose, triglyceride, leptin and non-esterified fatty acid (NEFA) levels as well as morphometric measurements such as body weight and the total fat following feeding with the CAF diet in the rat. These results show the importance of the genetic background in nutritional genomics and identify inbred rat strains as potential models for CAF-diet-induced obesity.© 2016. Published by The Company of Biologists Ltd.
Thematic Areas: Pathology Odontología Neuroscience (miscellaneous) Medicine (miscellaneous) Medicina veterinaria Medicina i Immunology and microbiology (miscellaneous) General biochemistry,genetics and molecular biology Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: neus.martinez@urv.cat anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat
Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://journals.biologists.com/dmm/article/9/10/1231/3814/Identification-of-a-nutrient-sensing
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Disease Models & Mechanisms. 9 (10): 1231-1239
APA: Martinez-Micaelo, Neus; Gonzalez-Abuin, Noemi; Terra, Ximena; Ardevol, Ana; Pinent, Montserrat; Petretto, Enrico; Behmoaras, Jacques; Blay, Mayte (2016). Identification of a nutrient-sensing transcriptional network in monocytes by using inbred rat models on a cafeteria diet. Disease Models & Mechanisms, 9(10), 1231-1239. DOI: 10.1242/dmm.025528
Article's DOI: 10.1242/dmm.025528
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV