Articles producció científica> Medicina i Cirurgia

Paraoxonase-1 Deficiency does not Influence Clopidogrel Antiplatelet Function in Mice

  • Identification data

    Identifier: imarina:5131370
    Authors:
    García-Heredia, A.Hernandez-Aguilera, A.Fort-Gallifa, I.Joven, J.Martín-Paredero, V.Camps, J
    Abstract:
    Abstract Background: Clopidogrel is an antiplatelet agent used in the treatment of vascular diseases. It requires in vivo bio activation linked to the cytochrome P450. Several studies reported that paraoxonase-1 (PON1) was a crucial enzyme in clopidogrel activation, and that patients carrying a variant of the PON1192 gene polymorphism have a high risk of thrombosis. However, these reports were not confirmed by subsequent results. The present study was aimed at investigating whether PON1 deficiency affects the biological action of clopidogrel in mice. Methods: PON1-deficient mice (n = 50) and wild type animals (n = 50) received different treatments for 3 days: a) clopidogrel, b) aspirin, c) cilostazol, d) clopidogrel + aspirin, and e) clopidogrel + aspirin + cilostazol. Blood was collected for the Platelet Function Analysis (PFA-100). Results: The different anticoagulant treatments resulted in higher aggregation times in all the mice, compared to the internal PFA control; demonstrating the anti-platelet effect of these compounds. We did not observe any significant alterations on the PFA assay in PON1-deficient mice, relative to wild type animals. Conclusion: PON1 deficiency does not influence the antiplatelet action of clopidogrel in mice, and supports the proposition that this enzyme is not involved in clopidogrel bio activation.
  • Others:

    Author, as appears in the article.: García-Heredia, A.; Hernandez-Aguilera, A.; Fort-Gallifa, I.; Joven, J.; Martín-Paredero, V.; Camps, J
    Department: Medicina i Cirurgia
    URV's Author/s: Camps Andreu, Jorge / Joven Maried, Jorge / Martín Paredero, Vicente
    Abstract: Abstract Background: Clopidogrel is an antiplatelet agent used in the treatment of vascular diseases. It requires in vivo bio activation linked to the cytochrome P450. Several studies reported that paraoxonase-1 (PON1) was a crucial enzyme in clopidogrel activation, and that patients carrying a variant of the PON1192 gene polymorphism have a high risk of thrombosis. However, these reports were not confirmed by subsequent results. The present study was aimed at investigating whether PON1 deficiency affects the biological action of clopidogrel in mice. Methods: PON1-deficient mice (n = 50) and wild type animals (n = 50) received different treatments for 3 days: a) clopidogrel, b) aspirin, c) cilostazol, d) clopidogrel + aspirin, and e) clopidogrel + aspirin + cilostazol. Blood was collected for the Platelet Function Analysis (PFA-100). Results: The different anticoagulant treatments resulted in higher aggregation times in all the mice, compared to the internal PFA control; demonstrating the anti-platelet effect of these compounds. We did not observe any significant alterations on the PFA assay in PON1-deficient mice, relative to wild type animals. Conclusion: PON1 deficiency does not influence the antiplatelet action of clopidogrel in mice, and supports the proposition that this enzyme is not involved in clopidogrel bio activation.
    Thematic Areas: Medicina ii Interdisciplinar Educação física Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: vicente.martin@urv.cat jorge.camps@urv.cat jorge.joven@urv.cat
    Author identifier: 0000-0002-2740-5847 0000-0002-3165-3640 0000-0003-2749-4541
    Record's date: 2024-02-03
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.walshmedicalmedia.com/open-access/paraoxonase1-deficiency-does-not-influence-clopidogrel-antiplatelet-function-in-mice-11909.html
    Papper original source: Cardiovasc Pharm Open Access. 4 (3): 1000148-
    APA: García-Heredia, A.; Hernandez-Aguilera, A.; Fort-Gallifa, I.; Joven, J.; Martín-Paredero, V.; Camps, J (2015). Paraoxonase-1 Deficiency does not Influence Clopidogrel Antiplatelet Function in Mice. Cardiovasc Pharm Open Access, 4(3), 1000148-. DOI: 10.4172/2329-6607.1000148
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.4172/2329-6607.1000148
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2015
    Publication Type: Journal Publications
  • Keywords:

    Medicina ii
    Interdisciplinar
    Educação física
    Biotecnología
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