Articles producció científica> Medicina i Cirurgia

Paraoxonase-1 Deficiency does not Influence Clopidogrel Antiplatelet Function in Mice

  • Datos identificativos

    Identificador: imarina:5131370
    Autores:
    García-Heredia, A.Hernandez-Aguilera, A.Fort-Gallifa, I.Joven, J.Martín-Paredero, V.Camps, J
    Resumen:
    Abstract Background: Clopidogrel is an antiplatelet agent used in the treatment of vascular diseases. It requires in vivo bio activation linked to the cytochrome P450. Several studies reported that paraoxonase-1 (PON1) was a crucial enzyme in clopidogrel activation, and that patients carrying a variant of the PON1192 gene polymorphism have a high risk of thrombosis. However, these reports were not confirmed by subsequent results. The present study was aimed at investigating whether PON1 deficiency affects the biological action of clopidogrel in mice. Methods: PON1-deficient mice (n = 50) and wild type animals (n = 50) received different treatments for 3 days: a) clopidogrel, b) aspirin, c) cilostazol, d) clopidogrel + aspirin, and e) clopidogrel + aspirin + cilostazol. Blood was collected for the Platelet Function Analysis (PFA-100). Results: The different anticoagulant treatments resulted in higher aggregation times in all the mice, compared to the internal PFA control; demonstrating the anti-platelet effect of these compounds. We did not observe any significant alterations on the PFA assay in PON1-deficient mice, relative to wild type animals. Conclusion: PON1 deficiency does not influence the antiplatelet action of clopidogrel in mice, and supports the proposition that this enzyme is not involved in clopidogrel bio activation.
  • Otros:

    Autor según el artículo: García-Heredia, A.; Hernandez-Aguilera, A.; Fort-Gallifa, I.; Joven, J.; Martín-Paredero, V.; Camps, J
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: Camps Andreu, Jorge / Joven Maried, Jorge / Martín Paredero, Vicente
    Resumen: Abstract Background: Clopidogrel is an antiplatelet agent used in the treatment of vascular diseases. It requires in vivo bio activation linked to the cytochrome P450. Several studies reported that paraoxonase-1 (PON1) was a crucial enzyme in clopidogrel activation, and that patients carrying a variant of the PON1192 gene polymorphism have a high risk of thrombosis. However, these reports were not confirmed by subsequent results. The present study was aimed at investigating whether PON1 deficiency affects the biological action of clopidogrel in mice. Methods: PON1-deficient mice (n = 50) and wild type animals (n = 50) received different treatments for 3 days: a) clopidogrel, b) aspirin, c) cilostazol, d) clopidogrel + aspirin, and e) clopidogrel + aspirin + cilostazol. Blood was collected for the Platelet Function Analysis (PFA-100). Results: The different anticoagulant treatments resulted in higher aggregation times in all the mice, compared to the internal PFA control; demonstrating the anti-platelet effect of these compounds. We did not observe any significant alterations on the PFA assay in PON1-deficient mice, relative to wild type animals. Conclusion: PON1 deficiency does not influence the antiplatelet action of clopidogrel in mice, and supports the proposition that this enzyme is not involved in clopidogrel bio activation.
    Áreas temáticas: Medicina ii Interdisciplinar Educação física Biotecnología
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: vicente.martin@urv.cat jorge.camps@urv.cat jorge.joven@urv.cat
    Identificador del autor: 0000-0002-2740-5847 0000-0002-3165-3640 0000-0003-2749-4541
    Fecha de alta del registro: 2024-02-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.walshmedicalmedia.com/open-access/paraoxonase1-deficiency-does-not-influence-clopidogrel-antiplatelet-function-in-mice-11909.html
    Referencia al articulo segun fuente origial: Cardiovasc Pharm Open Access. 4 (3): 1000148-
    Referencia de l'ítem segons les normes APA: García-Heredia, A.; Hernandez-Aguilera, A.; Fort-Gallifa, I.; Joven, J.; Martín-Paredero, V.; Camps, J (2015). Paraoxonase-1 Deficiency does not Influence Clopidogrel Antiplatelet Function in Mice. Cardiovasc Pharm Open Access, 4(3), 1000148-. DOI: 10.4172/2329-6607.1000148
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.4172/2329-6607.1000148
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2015
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Medicina ii
    Interdisciplinar
    Educação física
    Biotecnología
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