Author, as appears in the article.: Yuste, Silvia; Macia, Alba; Ludwig, Iziar A; Romero, Maria-Paz; Fernandez-Castillejo, Sara; Catalan, Ursula; Motilva, Maria-Jose; Rubio, Laura
Department: Medicina i Cirurgia
URV's Author/s: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA
Keywords: Whole blood Tandem mass spectrometry Solvents Solid phase extraction Plasma samples Plasma Phlebotomy Phenols Phenolic metabolites Ms/ms Middle aged Malus Male Limit of detection Humans Fruit Female Dried blood spot testing Dried blood spot cards Chromatography, high pressure liquid Adult plasma phenolic metabolites ms/ms dried blood spot cards
Abstract: SCOPE: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. METHODS AND RESULTS: An analytical method based on blood sampling with DBS cards combined with LC-MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (µSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-¿-valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or µSPE. CONCLUSIONS: Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.
Thematic Areas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 16134125
Author's mail: ursula.catalan@urv.cat
Author identifier: 0000-0001-8884-9823
Record's date: 2025-03-03
Paper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Molecular Nutrition & Food Research. 62 (23): e1800623-
APA: Yuste, Silvia; Macia, Alba; Ludwig, Iziar A; Romero, Maria-Paz; Fernandez-Castillejo, Sara; Catalan, Ursula; Motilva, Maria-Jose; Rubio, Laura (2018). Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red-Fleshed Apple Snack. Molecular Nutrition & Food Research, 62(23), e1800623-. DOI: 10.1002/mnfr.201800623
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV