Autor según el artículo: Yuste S., Macià A., Ludwig I., Romero M., Fernández-Castillejo S., Catalán Ú., Motilva M., Rubió L.
Departamento: Medicina i Cirurgia
Autor/es de la URV: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA
Palabras clave: Whole blood Plasma samples Plasma Phenolic metabolites Ms/ms Dried blood spot cards plasma phenolic metabolites ms/ms dried blood spot cards
Resumen: SCOPE: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. METHODS AND RESULTS: An analytical method based on blood sampling with DBS cards combined with LC-MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (µSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-¿-valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or µSPE. CONCLUSIONS: Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.
Áreas temáticas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 16134125
Direcció de correo del autor: ursula.catalan@urv.cat
Identificador del autor: 0000-0001-8884-9823
Fecha de alta del registro: 2024-09-07
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/epdf/10.1002/mnfr.201800623
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Molecular Nutrition & Food Research. 62 (23): e1800623-
Referencia de l'ítem segons les normes APA: Yuste S., Macià A., Ludwig I., Romero M., Fernández-Castillejo S., Catalán Ú., Motilva M., Rubió L. (2018). Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red-Fleshed Apple Snack. Molecular Nutrition & Food Research, 62(23), e1800623-. DOI: 10.1002/mnfr.201800623
DOI del artículo: 10.1002/mnfr.201800623
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2018
Tipo de publicación: Journal Publications
Repositori URV