Author, as appears in the article.: Javier Lozano Bartolomé; Gemma Llauradó; Manel Portero Otin; Antonio Altuna Coy; Gemma Rojo Martínez; Joan Vendrell; Rosa Jorba; Esther Rodríguez Gallego; Matilde Rodríguez-Chacón
Department: Medicina i Cirurgia Bioquímica i Biotecnologia
URV's Author/s: Jorba Martin, Rosa Maria / Vendrell Ortega, Juan José
Keywords: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu
Abstract: The proinflammatory cytokine TNF? is a key player in insulin resistance (IR). The role of miRNAs in inflammation associated with IR is poorly understood.To investigate miR-181a-5p and miR-23a-3p expression profiles in obesity and to study their role in TNF?-induced IR in adipocytes.Two separate cohorts were used. Cohort 1 was used in adipose tissue (AT) expression studies and included 28 subjects with body mass index (BMI) <30 kg/m2 and 30 with BMI ?30 kg/m2. Cohort 2 was used in circulating serum miRNA studies and included 101 subjects with 4 years of follow-up (48 case subjects and 53 control subjects). miR-181a-5p and miR-23a-3p expression was assessed in subcutaneous and visceral AT. Functional analysis was performed in adipocytes, using miRNA mimics and inhibitors. Key molecules of the insulin pathway, AKT, PTEN, AS160, and S6K, were analyzed.Expression of miR-181a-5p and miR-23a-3p was reduced in adipose tissue from obese and diabetic subjects and was inversely correlated to adiposity and homeostasis model assessment of IR index. Overexpression of miR-181a-5p and miR-23a-3p in adipocytes upregulated insulin-stimulated AKT activation and reduced TNF?-induced IR, regulating PTEN and S6K expression. Serum levels of miR-181a-5p were reduced in case vs control subjects at baseline, suggesting a prognostic value. Variable importance in projection scores revealed miR-181a-5p had more effect on the model than insulin or glucose at 120 minutes.miR-181a-5p and miR-23a-3p may prevent TNF?-induced IR in adipocytes through modulation of PTEN and S6K expression.
Thematic Areas: Sociología Saúde coletiva Odontología Nutrição Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias ii Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry (medical) Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0021972X
Author's mail: juanjose.vendrell@urv.cat rosamaria.jorba@urv.cat
Author identifier: 0000-0002-6994-6115 0000-0003-3307-4340
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://academic.oup.com/jcem/article/103/4/1447/4834033
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Clinical Endocrinology & Metabolism. 103 (4): 1447-1458
APA: Javier Lozano Bartolomé; Gemma Llauradó; Manel Portero Otin; Antonio Altuna Coy; Gemma Rojo Martínez; Joan Vendrell; Rosa Jorba; Esther Rodríguez Gall (2018). Altered Expression of miR-181a-5p and miR-23a-3p Is Associated With Obesity and TNFα-Induced Insulin Resistance. Journal Of Clinical Endocrinology & Metabolism, 103(4), 1447-1458. DOI: 10.1210/jc.2017-01909
Article's DOI: 10.1210/jc.2017-01909
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications