Autor según el artículo: Javier Lozano Bartolomé; Gemma Llauradó; Manel Portero Otin; Antonio Altuna Coy; Gemma Rojo Martínez; Joan Vendrell; Rosa Jorba; Esther Rodríguez Gallego; Matilde Rodríguez-Chacón

Departamento: Medicina i Cirurgia Bioquímica i Biotecnologia

Autor/es de la URV: Jorba Martin, Rosa Maria / Vendrell Ortega, Juan José

Palabras clave: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu

Resumen: The proinflammatory cytokine TNF? is a key player in insulin resistance (IR). The role of miRNAs in inflammation associated with IR is poorly understood.To investigate miR-181a-5p and miR-23a-3p expression profiles in obesity and to study their role in TNF?-induced IR in adipocytes.Two separate cohorts were used. Cohort 1 was used in adipose tissue (AT) expression studies and included 28 subjects with body mass index (BMI) <30 kg/m2 and 30 with BMI ?30 kg/m2. Cohort 2 was used in circulating serum miRNA studies and included 101 subjects with 4 years of follow-up (48 case subjects and 53 control subjects). miR-181a-5p and miR-23a-3p expression was assessed in subcutaneous and visceral AT. Functional analysis was performed in adipocytes, using miRNA mimics and inhibitors. Key molecules of the insulin pathway, AKT, PTEN, AS160, and S6K, were analyzed.Expression of miR-181a-5p and miR-23a-3p was reduced in adipose tissue from obese and diabetic subjects and was inversely correlated to adiposity and homeostasis model assessment of IR index. Overexpression of miR-181a-5p and miR-23a-3p in adipocytes upregulated insulin-stimulated AKT activation and reduced TNF?-induced IR, regulating PTEN and S6K expression. Serum levels of miR-181a-5p were reduced in case vs control subjects at baseline, suggesting a prognostic value. Variable importance in projection scores revealed miR-181a-5p had more effect on the model than insulin or glucose at 120 minutes.miR-181a-5p and miR-23a-3p may prevent TNF?-induced IR in adipocytes through modulation of PTEN and S6K expression.

Áreas temáticas: Sociología Saúde coletiva Odontología Nutrição Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias ii Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry (medical) Biochemistry

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0021972X

Direcció de correo del autor: rosamaria.jorba@urv.cat juanjose.vendrell@urv.cat

Identificador del autor: 0000-0003-3307-4340 0000-0002-6994-6115

Fecha de alta del registro: 2023-02-22

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://academic.oup.com/jcem/article/103/4/1447/4834033

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Journal Of Clinical Endocrinology & Metabolism. 103 (4): 1447-1458

Referencia de l'ítem segons les normes APA: Javier Lozano Bartolomé; Gemma Llauradó; Manel Portero Otin; Antonio Altuna Coy; Gemma Rojo Martínez; Joan Vendrell; Rosa Jorba; Esther Rodríguez Gall (2018). Altered Expression of miR-181a-5p and miR-23a-3p Is Associated With Obesity and TNFα-Induced Insulin Resistance. Journal Of Clinical Endocrinology & Metabolism, 103(4), 1447-1458. DOI: 10.1210/jc.2017-01909

DOI del artículo: 10.1210/jc.2017-01909

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2018

Tipo de publicación: Journal Publications