A trimer plus a dimer-gallate reproduce the bioactivity described for an extract of grape seed procyanidins

Identifier:  imarina:6385967

Handle: https://hdl.handle.net/20.500.11797/imarina6385967

Authors:  Montagut, Gemma; Baiges, Isabel; Valls, Josep; Terra, Ximena; del Bas, Josep M; Vitrac, Xavier; Richard, Tristan; Merillon, Jean-Michel; Arola, Lluis; Blay, Mayte; Blade, Cinta; Fernandez-Larrea, Juan; Pujadas, Gerard; Salvado, Josepa; Ardevol, Anna

Abstract:
The relationship between grape seed-derived procyanidin extract components and their bioactivity was explored. The monomeric and dimeric structures only acted as anti-inflammatory agents. Similarly, pure C1 trimer was highly effective on LPS-activated macrophages. To reproduce all of the bioactivities of the total extract, a fraction enriched with trimeric structures was needed. This trimeric-enriched fraction was divided into subfractions, the most bioactive of which contained two compounds with a molecular weight equal to a trimer (865) and a dimer-gallate (729), according to spectrometric analysis. Thus, it may be concluded that a mixture of both molecules reproduces the bioactivity in glucose metabolism (3T3-L1), lipid metabolism (HepG2) and macrophage functionality (RAW 264.6). © 2009 Elsevier Ltd. All rights reserved.