Author, as appears in the article.: Mottola A; Bonerba E; Figueras M; Pérez-Cataluña A; Marchetti P; Serraino A; Bozzo G; Terio V; Tantillo G; Di Pinto A
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Figueras Salvat, María Josefa / PÉREZ CATALUÑA, ALBA
Keywords: arcobacter bivalve molluscs food-borne pathogen Arcobacter Bivalve molluscs Food-borne pathogen Putative virulence factors
Abstract: © 2016 Elsevier Ltd. Considering that several recent cases of human gastroenteritis have been associated with species from the Arcobacter genus, and that few data are currently available about the occurrence of this genus in Italian shellfish, the aim of the present study was to evaluate the occurrence of Arcobacter spp. and the presence of virulence-associated genes. The approach consisted of cultural and biomolecular (multiplex-PCR and 16S-RFLP) methods identifying isolates, followed by PCR assays aimed at the cadF, ciaB, cjl349, irgA, hecA putative virulence genes. Arcobacter spp. was detected in 16/70 (22.8%) shellfish samples. Specifically, Arcobacter spp. was highlighted in 10/42 (23.8%) mussel and in 6/28 (21.4%) clam samples. Subsequently, biomolecular assays revealed Arcobacter butzleri in 12/16 (75%) and Arcobacter cryaerophilus 1B in 4/16 (25%) isolates. PCRs aimed at the five putative virulence genes demonstrated widespread distribution of these genes among Arcobacter isolates and some differences from the results published by other authors. Our research provides more information regarding the health risks associated with the consumption of raw bivalve molluscs and underlines the need to implement an adequate control plan by performing intensive and continuous monitoring in order to guarantee human health.
Thematic Areas: Biodiversidade Biotechnology & applied microbiology Biotecnología Ciência de alimentos Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Engenharias ii Farmacia Food science Food science & technology Medicina i Medicina veterinaria Microbiology Nutrição Química Zootecnia / recursos pesqueiros
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariajose.figueras@urv.cat
ISSN: 07400020
Author identifier: 0000-0002-2268-8980
Record's date: 2023-02-26
Papper version: info:eu-repo/semantics/submittedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0740002015300198?via%3Dihub
Papper original source: Food Microbiology. 57 23-27
APA: Mottola A; Bonerba E; Figueras M; Pérez-Cataluña A; Marchetti P; Serraino A; Bozzo G; Terio V; Tantillo G; Di Pinto A (2016). Occurrence of potentially pathogenic arcobacters in shellfish. Food Microbiology, 57(), 23-27. DOI: 10.1016/j.fm.2015.12.010
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1016/j.fm.2015.12.010
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV