Articles producció científica> Ciències Mèdiques Bàsiques

Occurrence of potentially pathogenic arcobacters in shellfish

  • Datos identificativos

    Identificador: imarina:6388509
    Autores:
    Mottola ABonerba EFigueras MPérez-Cataluña AMarchetti PSerraino ABozzo GTerio VTantillo GDi Pinto A
    Resumen:
    © 2016 Elsevier Ltd. Considering that several recent cases of human gastroenteritis have been associated with species from the Arcobacter genus, and that few data are currently available about the occurrence of this genus in Italian shellfish, the aim of the present study was to evaluate the occurrence of Arcobacter spp. and the presence of virulence-associated genes. The approach consisted of cultural and biomolecular (multiplex-PCR and 16S-RFLP) methods identifying isolates, followed by PCR assays aimed at the cadF, ciaB, cjl349, irgA, hecA putative virulence genes. Arcobacter spp. was detected in 16/70 (22.8%) shellfish samples. Specifically, Arcobacter spp. was highlighted in 10/42 (23.8%) mussel and in 6/28 (21.4%) clam samples. Subsequently, biomolecular assays revealed Arcobacter butzleri in 12/16 (75%) and Arcobacter cryaerophilus 1B in 4/16 (25%) isolates. PCRs aimed at the five putative virulence genes demonstrated widespread distribution of these genes among Arcobacter isolates and some differences from the results published by other authors. Our research provides more information regarding the health risks associated with the consumption of raw bivalve molluscs and underlines the need to implement an adequate control plan by performing intensive and continuous monitoring in order to guarantee human health.
  • Otros:

    Autor según el artículo: Mottola A; Bonerba E; Figueras M; Pérez-Cataluña A; Marchetti P; Serraino A; Bozzo G; Terio V; Tantillo G; Di Pinto A
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Figueras Salvat, María Josefa / PÉREZ CATALUÑA, ALBA
    Palabras clave: Putative virulence factors Food-borne pathogen Bivalve molluscs Arcobacter food-borne pathogen bivalve molluscs arcobacter
    Resumen: © 2016 Elsevier Ltd. Considering that several recent cases of human gastroenteritis have been associated with species from the Arcobacter genus, and that few data are currently available about the occurrence of this genus in Italian shellfish, the aim of the present study was to evaluate the occurrence of Arcobacter spp. and the presence of virulence-associated genes. The approach consisted of cultural and biomolecular (multiplex-PCR and 16S-RFLP) methods identifying isolates, followed by PCR assays aimed at the cadF, ciaB, cjl349, irgA, hecA putative virulence genes. Arcobacter spp. was detected in 16/70 (22.8%) shellfish samples. Specifically, Arcobacter spp. was highlighted in 10/42 (23.8%) mussel and in 6/28 (21.4%) clam samples. Subsequently, biomolecular assays revealed Arcobacter butzleri in 12/16 (75%) and Arcobacter cryaerophilus 1B in 4/16 (25%) isolates. PCRs aimed at the five putative virulence genes demonstrated widespread distribution of these genes among Arcobacter isolates and some differences from the results published by other authors. Our research provides more information regarding the health risks associated with the consumption of raw bivalve molluscs and underlines the need to implement an adequate control plan by performing intensive and continuous monitoring in order to guarantee human health.
    Áreas temáticas: Zootecnia / recursos pesqueiros Química Nutrição Microbiology Medicina veterinaria Medicina i Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biodiversidade
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 07400020
    Direcció de correo del autor: mariajose.figueras@urv.cat
    Identificador del autor: 0000-0002-2268-8980
    Fecha de alta del registro: 2024-08-31
    Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S0740002015300198?via%3Dihub
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Food Microbiology. 57 23-27
    Referencia de l'ítem segons les normes APA: Mottola A; Bonerba E; Figueras M; Pérez-Cataluña A; Marchetti P; Serraino A; Bozzo G; Terio V; Tantillo G; Di Pinto A (2016). Occurrence of potentially pathogenic arcobacters in shellfish. Food Microbiology, 57(), 23-27. DOI: 10.1016/j.fm.2015.12.010
    DOI del artículo: 10.1016/j.fm.2015.12.010
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biotechnology & Applied Microbiology,Food Science,Food Science & Technology,Microbiology
    Putative virulence factors
    Food-borne pathogen
    Bivalve molluscs
    Arcobacter
    food-borne pathogen
    bivalve molluscs
    arcobacter
    Zootecnia / recursos pesqueiros
    Química
    Nutrição
    Microbiology
    Medicina veterinaria
    Medicina i
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biotechnology & applied microbiology
    Biodiversidade
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