Author, as appears in the article.: Latif-Eugenín F; Beaz-Hidalgo R; Figueras M
Department: Ciències Mèdiques Bàsiques
URV's Author/s: BEAZ HIDALGO, ROXANA / Figueras Salvat, María Josefa
Keywords: Water Shellfish Enrichment Direct plating Culture media Aeromonas shellfish enrichment direct plating culture media aeromonas
Abstract: © 2016 The Society for Applied Microbiology Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. Methods and Results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. Significance and Impact of the Study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Engenharias i Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biotechnology Biodiversidade Astronomia / física Applied microbiology and biotechnology Administração pública e de empresas, ciências contábeis e turismo
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 13645072
Author's mail: mariajose.figueras@urv.cat
Author identifier: 0000-0002-2268-8980
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.13210
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Applied Microbiology. 121 (3): 883-891
APA: Latif-Eugenín F; Beaz-Hidalgo R; Figueras M (2016). Evaluation of different conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples. Journal Of Applied Microbiology, 121(3), 883-891. DOI: 10.1111/jam.13210
Article's DOI: 10.1111/jam.13210
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV